• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

人食管癌组织与癌旁正常组织的蛋白质组比较分析

Comparative proteome analysis of human esophageal cancer and adjacent normal tissues.

作者信息

Yazdian-Robati Rezvan, Ahmadi Homa, Riahi Maryam Matbou, Lari Parisa, Aledavood Seyed Amir, Rashedinia Marzieh, Abnous Khalil, Ramezani Mohammad

机构信息

Department of Pharmaceutical Biotechnology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.

Department of Pharmacodynamics and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.

出版信息

Iran J Basic Med Sci. 2017 Mar;20(3):265-271. doi: 10.22038/ijbms.2017.8354.

DOI:10.22038/ijbms.2017.8354
PMID:28392898
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5378963/
Abstract

OBJECTIVES

Ranking as the sixth commonest cancer, esophageal squamous cell carcinoma (ESCC) represents one of the leading causes of cancer death worldwide. One of the main reasons for the low survival of patients with esophageal cancer is its late diagnosis.

MATERIALS AND METHODS

We used proteomics approach to analyze ESCC tissues with the aim of a better understanding of the malignant mechanism and searching candidate protein biomarkers for early diagnosis of esophageal cancer. The differential protein expression between cancerous and normal esophageal tissues was investigated by two-dimensional polyacrylamide gel electrophoresis (2D-PAGE). Then proteins were identified by matrix-assisted laser desorption/ ionization tandem time-of-flight mass spectrometry (MALDI-TOF/TOF-MS) and MASCOT web based search engine.

RESULTS

We reported 4 differentially expressed proteins involved in the pathological process of esophageal cancer, such as annexinA1 (ANXA1), peroxiredoxin-2 (PRDX2), transgelin (TAGLN) andactin-aortic smooth muscle (ACTA2).

CONCLUSION

In this report we have introduced new potential biomarker (ACTA2). Moreover, our data confirmed some already known markers for EC in our region.

摘要

目的

食管鳞状细胞癌（ESCC）是全球第六大常见癌症，也是全球癌症死亡的主要原因之一。食管癌患者生存率低的主要原因之一是其诊断较晚。

材料与方法

我们采用蛋白质组学方法分析ESCC组织，旨在更好地了解其恶性机制，并寻找用于食管癌早期诊断的候选蛋白质生物标志物。通过二维聚丙烯酰胺凝胶电泳（2D-PAGE）研究癌组织与正常食管组织之间的差异蛋白质表达。然后通过基质辅助激光解吸/电离串联飞行时间质谱（MALDI-TOF/TOF-MS）和基于MASCOT的网络搜索引擎鉴定蛋白质。

结果

我们报告了4种参与食管癌病理过程的差异表达蛋白质，如膜联蛋白A1（ANXA1）、过氧化物酶还原蛋白2（PRDX2）、转胶蛋白（TAGLN）和肌动蛋白-主动脉平滑肌（ACTA2）。

结论

在本报告中，我们介绍了新的潜在生物标志物（ACTA2）。此外，我们的数据证实了我们所在地区一些已知用于食管癌的标志物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3aae/5378963/0549c50b4873/IJBMS-20-265-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3aae/5378963/f4df8d90f7f9/IJBMS-20-265-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3aae/5378963/8c616abfcb78/IJBMS-20-265-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3aae/5378963/0549c50b4873/IJBMS-20-265-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3aae/5378963/f4df8d90f7f9/IJBMS-20-265-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3aae/5378963/8c616abfcb78/IJBMS-20-265-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3aae/5378963/0549c50b4873/IJBMS-20-265-g003.jpg

相似文献

1
Comparative proteome analysis of human esophageal cancer and adjacent normal tissues.人食管癌组织与癌旁正常组织的蛋白质组比较分析
Iran J Basic Med Sci. 2017 Mar;20(3):265-271. doi: 10.22038/ijbms.2017.8354.
2
Using proteomic approach to identify tumor-associated proteins as biomarkers in human esophageal squamous cell carcinoma.采用蛋白质组学方法鉴定人食管鳞癌相关肿瘤标志物。
J Proteome Res. 2011 Jun 3;10(6):2863-72. doi: 10.1021/pr200141c. Epub 2011 May 3.
3
Identification of potential plasma biomarkers for esophageal squamous cell carcinoma by a proteomic method.通过蛋白质组学方法鉴定食管鳞状细胞癌潜在的血浆生物标志物
Int J Clin Exp Pathol. 2015 Feb 1;8(2):1535-44. eCollection 2015.
4
Identification of PA28β as a potential novel biomarker in human esophageal squamous cell carcinoma.鉴定PA28β作为人食管鳞状细胞癌潜在的新型生物标志物。
Tumour Biol. 2017 Oct;39(10):1010428317719780. doi: 10.1177/1010428317719780.
5
Comparative proteomic analysis of esophageal squamous cell carcinoma.食管鳞状细胞癌的比较蛋白质组学分析
Proteomics. 2005 Jul;5(11):2960-71. doi: 10.1002/pmic.200401175.
6
[Screening of differentially expressed proteins from human esophageal cancer and esophageal tissues by two-dimensional difference gel electrophoresis and mass spectrometry].[应用二维差异凝胶电泳和质谱技术筛选人食管癌组织与食管组织差异表达蛋白质]
Nan Fang Yi Ke Da Xue Xue Bao. 2007 Sep;27(9):1406-9.
7
Proteomic analysis to identify biomarker proteins in pancreatic ductal adenocarcinoma.蛋白质组学分析以鉴定胰腺导管腺癌中的生物标志物蛋白。
ANZ J Surg. 2008 Apr;78(4):245-51. doi: 10.1111/j.1445-2197.2008.04429.x.
8
Proteomics-based approach identifying autoantibody against peroxiredoxin VI as a novel serum marker in esophageal squamous cell carcinoma.基于蛋白质组学的方法鉴定抗过氧化物酶体增殖物激活受体VI自身抗体作为食管鳞状细胞癌的一种新型血清标志物。
Clin Cancer Res. 2006 Nov 1;12(21):6415-20. doi: 10.1158/1078-0432.CCR-06-1315.
9
Under-expression of annexin A2 is associated with Kazakh's esophageal squamous cell carcinoma.膜联蛋白A2表达下调与哈萨克族食管鳞状细胞癌相关。
Mol Carcinog. 2015 Sep;54(9):779-88. doi: 10.1002/mc.22145. Epub 2014 Mar 26.
10
Comparative proteome analysis of breast cancer and adjacent normal breast tissues in human.人乳腺癌组织与癌旁正常乳腺组织的蛋白质组比较分析。
Genomics Proteomics Bioinformatics. 2006 Aug;4(3):165-72. doi: 10.1016/S1672-0229(06)60029-6.

引用本文的文献

1
A metabolomic and proteomic study to elucidate the molecular mechanisms of immunotherapy resistance in patients with oesophageal squamous cell carcinoma.一项代谢组学和蛋白质组学研究，以阐明食管鳞状细胞癌患者免疫治疗耐药的分子机制。
Biomed Rep. 2023 Apr 6;18(5):36. doi: 10.3892/br.2023.1619. eCollection 2023 May.
2
Integrative proteogenomic characterization of early esophageal cancer.早期食管癌的综合蛋白质基因组学特征分析。
Nat Commun. 2023 Mar 25;14(1):1666. doi: 10.1038/s41467-023-37440-w.
3
Urinary protein biomarker panel predicts esophageal squamous carcinoma from control cases and other tumors.

本文引用的文献

1
α-Smooth muscle actin expression and desmoplastic stromal reaction in pancreatic cancer: results from the CONKO-001 study.胰腺癌中α平滑肌肌动蛋白的表达及促纤维增生性间质反应：CONKO-001研究结果
Br J Cancer. 2014 Nov 11;111(10):1917-23. doi: 10.1038/bjc.2014.495. Epub 2014 Oct 14.
2
Current advances in esophageal cancer proteomics.食管癌蛋白质组学的当前进展
Biochim Biophys Acta. 2015 Jun;1854(6):687-95. doi: 10.1016/j.bbapap.2014.09.011. Epub 2014 Sep 16.
3
Differential expression of ANXA1 in benign human gastrointestinal tissues and cancers.
尿蛋白生物标志物谱可预测食管鳞癌与对照病例和其他肿瘤。
Esophagus. 2022 Oct;19(4):604-616. doi: 10.1007/s10388-022-00932-7. Epub 2022 Jul 6.
4
Integrative proteomic characterization of trace FFPE samples in early-stage gastrointestinal cancer.早期胃肠道癌微量福尔马林固定石蜡包埋样本的综合蛋白质组学特征分析
Proteome Sci. 2022 Apr 9;20(1):5. doi: 10.1186/s12953-022-00188-0.
5
Identification of key genes associated with esophageal adenocarcinoma based on bioinformatics analysis.基于生物信息学分析鉴定与食管腺癌相关的关键基因
Ann Transl Med. 2021 Dec;9(23):1711. doi: 10.21037/atm-21-4015.
6
Protective Effect of Crocin on Malathion-induced Cardiotoxicity in Rats: A Biochemical, Histopathological and Proteomics Study.西红花苷对马拉硫磷诱导的大鼠心脏毒性的保护作用：一项生物化学、组织病理学和蛋白质组学研究
Iran J Pharm Res. 2021 Spring;20(2):156-172. doi: 10.22037/ijpr.2020.111836.13385.
7
Comparative proteomics study of proteins involved in induction of higher rates of cell death in mitoxantrone-resistant breast cancer cells MCF-7/MX exposed to TNF-α.对暴露于肿瘤坏死因子-α（TNF-α）的米托蒽醌耐药乳腺癌细胞MCF-7/MX中参与诱导更高细胞死亡率的蛋白质进行的比较蛋白质组学研究。
Iran J Basic Med Sci. 2020 May;23(5):663-672. doi: 10.22038/ijbms.2020.40029.9486.
膜联蛋白A1（ANXA1）在人胃肠道良性组织和癌症中的差异表达。
BMC Cancer. 2014 Jul 19;14:520. doi: 10.1186/1471-2407-14-520.
4
Transgelins, cytoskeletal proteins implicated in different aspects of cancer development.转胶蛋白，细胞骨架蛋白，涉及癌症发展的多个方面。
Expert Rev Proteomics. 2014 Apr;11(2):149-65. doi: 10.1586/14789450.2014.860358. Epub 2014 Jan 29.
5
Alpha-smooth muscle actin (ACTA2) is required for metastatic potential of human lung adenocarcinoma.α-平滑肌肌动蛋白（ACTA2）对于人肺腺癌的转移潜能是必需的。
Clin Cancer Res. 2013 Nov 1;19(21):5879-89. doi: 10.1158/1078-0432.CCR-13-1181. Epub 2013 Aug 30.
6
An overview of esophageal squamous cell carcinoma proteomics.食管鳞状细胞癌蛋白质组学概述。
J Proteomics. 2012 Jun 18;75(11):3129-37. doi: 10.1016/j.jprot.2012.04.025. Epub 2012 Apr 27.
7
Epidemiologic differences in esophageal cancer between Asian and Western populations.亚洲和西方人群食管癌的流行病学差异。
Chin J Cancer. 2012 Jun;31(6):281-6. doi: 10.5732/cjc.011.10390. Epub 2012 Apr 13.
8
Novel roles of peroxiredoxins in inflammation, cancer and innate immunity.过氧化物酶在炎症、癌症和先天免疫中的新作用。
J Clin Biochem Nutr. 2012 Mar;50(2):91-105. doi: 10.3164/jcbn.11-109. Epub 2012 Feb 18.
9
Proteomics of a new esophageal cancer cell line established from Persian patient.从波斯患者中建立的新型食管癌细胞系的蛋白质组学研究。
Gene. 2012 May 25;500(1):124-33. doi: 10.1016/j.gene.2012.03.038. Epub 2012 Mar 14.
10
Quantitative tissue proteomics of esophageal squamous cell carcinoma for novel biomarker discovery.食管鳞癌定量组织蛋白质组学用于新的生物标志物发现。
Cancer Biol Ther. 2011 Sep 15;12(6):510-22. doi: 10.4161/cbt.12.6.16833.