Morii S, Naka Y, Inui T, Shintani H
Department of Pathology, Kansai Medical University, Osaka, Japan.
Cancer Res. 1988 Aug 1;48(15):4395-8.
Thirty 18-month-old male F344/DuCrj rats were divided into the following groups: 10 untreated controls; eight vehicle-injected controls; and 12 ethanedimethanesulfonate (EDS)-injected rats. Untreated controls were killed immediately to check for testicular tumor incidence. In rats of the test group, a 75-mg/kg dose of EDS dissolved in dimethyl sulfoxide:water (1:3) was injected i.p. At intervals of 1, 2, 3, and 10 days after injection, two vehicle-injected control rats and three EDS-injected rats were sacrificed, and the testes were fixed by vascular perfusion. The midsagittal sections of all the fixed testes were examined to determine the incidence of macroscopic Leydig cell tumors, and some tumor tissues of the injection-treated groups were also investigated ultrastructurally. In 28 of 30 animals, a total of 78 Leydig cell tumors could be distinguished. Extensive and severe necrotic alterations accompanying fresh, multiple hemorrhages in early stages and reparative changes in later stages could be observed in a total of 78% of the 32 tumors examined from the EDS-injected group. The tumor cells exhibited ultrastructurally degenerative changes such as chromatin condensation and cytoplasmic vacuolation from 1 day after EDS injection. Therefore, EDS may be a necrotic agent for rat Leydig cell tumor.
将30只18个月大的雄性F344/DuCrj大鼠分为以下几组：10只未处理的对照组；8只注射赋形剂的对照组；以及12只注射乙烷二甲磺酸盐（EDS）的大鼠。立即处死未处理的对照组以检查睾丸肿瘤发生率。在试验组大鼠中，将75mg/kg剂量的EDS溶于二甲亚砜：水（1:3）中腹腔注射。在注射后的第1、2、3和10天，处死2只注射赋形剂的对照大鼠和3只注射EDS的大鼠，并通过血管灌注固定睾丸。检查所有固定睾丸的正中矢状切片以确定肉眼可见的Leydig细胞瘤的发生率，并且还对注射治疗组的一些肿瘤组织进行了超微结构研究。在30只动物中的28只中，总共可分辨出78个Leydig细胞瘤。在注射EDS组检查的32个肿瘤中，总共78%的肿瘤在早期观察到广泛而严重的坏死改变，并伴有新鲜的多处出血，在后期观察到修复性改变。从EDS注射后第1天起，肿瘤细胞在超微结构上表现出退行性变化，如染色质浓缩和细胞质空泡化。因此，EDS可能是大鼠Leydig细胞瘤的一种坏死剂。
