Yan Ting-Liang, Han Dun-Xin, Hu Jiang, Huang Xiao-Yun, Wang Hong-Kun
a Department of Basic Medicine , Qujing Medical College , Qujing 655000 , China.
b Department of Spine Surgery , 107th Hospital of People's Liberation Army , Yantai , China.
J Asian Nat Prod Res. 2017 Jun;19(6):550-556. doi: 10.1080/10286020.2017.1313242. Epub 2017 Apr 9.
Phytochemical investigation on the 70% ethanol extract of the leaves of Alstonia mairei resulted in the isolation of three new monoterpenoid indole alkaloids, alstomairines A-C (1-3), along with one known compound, alpneumine A (4). Structural elucidation of all the compounds was accomplished by spectral methods such as 1D and 2D NMR, IR, UV, and HRESIMS. The isolated compounds were tested in vitro for cytotoxic activities against four osteosarcoma cell lines. Consequently, alkaloids 2 and 3 exhibited cytotoxic activities for all tested tumor cell lines with IC values from 9.2 to 13.0 μM.
对鸡骨常山（Alstonia mairei）叶的70%乙醇提取物进行植物化学研究，从中分离出三种新的单萜吲哚生物碱，鸡骨常山碱A - C（1 - 3），以及一种已知化合物，高山远志碱A（4）。通过一维和二维核磁共振、红外光谱、紫外光谱和高分辨电喷雾电离质谱等光谱方法完成了所有化合物的结构解析。对分离得到的化合物进行体外抗四种骨肉瘤细胞系的细胞毒活性测试。结果表明，生物碱2和3对所有测试的肿瘤细胞系均表现出细胞毒活性，IC值为9.2至13.0 μM。
