Harterink Martin, da Silva Marta Esteves, Will Lena, Turan Julia, Ibrahim Adiljan, Lang Alexander E, van Battum Eljo Y, Pasterkamp R Jeroen, Kapitein Lukas C, Kudryashov Dmitri, Barres Ben A, Hoogenraad Casper C, Zuchero J Bradley
Cell Biology, Department of Biology, Faculty of Science, Utrecht University, Utrecht, the Netherlands.
Department of Neurobiology, Stanford University School of Medicine, Stanford, California, USA.
Nat Methods. 2017 May;14(5):479-482. doi: 10.1038/nmeth.4257. Epub 2017 Apr 10.
The actin cytoskeleton is essential for many fundamental biological processes, but tools for directly manipulating actin dynamics are limited to cell-permeable drugs that preclude single-cell perturbations. Here we describe DeActs, genetically encoded actin-modifying polypeptides, which effectively induce actin disassembly in eukaryotic cells. We demonstrate that DeActs are universal tools for studying the actin cytoskeleton in single cells in culture, tissues, and multicellular organisms including various neurodevelopmental model systems.
肌动蛋白细胞骨架对许多基本生物学过程至关重要，但直接操纵肌动蛋白动力学的工具仅限于可穿透细胞的药物，这些药物排除了单细胞扰动。在这里，我们描述了DeActs，即基因编码的肌动蛋白修饰多肽，它能有效诱导真核细胞中的肌动蛋白解聚。我们证明，DeActs是用于研究培养的单细胞、组织以及包括各种神经发育模型系统在内的多细胞生物体中肌动蛋白细胞骨架的通用工具。
