生命荧光蛋白：一种用于可视化丝状肌动蛋白的多功能标记物。
Lifeact: a versatile marker to visualize F-actin.
作者信息
Riedl Julia, Crevenna Alvaro H, Kessenbrock Kai, Yu Jerry Haochen, Neukirchen Dorothee, Bista Michal, Bradke Frank, Jenne Dieter, Holak Tad A, Werb Zena, Sixt Michael, Wedlich-Soldner Roland
机构信息
Max Planck Institute of Biochemistry, Independent Junior Research Group Cellular Dynamics and Cell Patterning, Am Klopferspitz 18, 82152 Martinsried, Germany.
出版信息
Nat Methods. 2008 Jul;5(7):605-7. doi: 10.1038/nmeth.1220. Epub 2008 Jun 8.
Abstract
Live imaging of the actin cytoskeleton is crucial for the study of many fundamental biological processes, but current approaches to visualize actin have several limitations. Here we describe Lifeact, a 17-amino-acid peptide, which stained filamentous actin (F-actin) structures in eukaryotic cells and tissues. Lifeact did not interfere with actin dynamics in vitro and in vivo and in its chemically modified peptide form allowed visualization of actin dynamics in nontransfectable cells.
摘要
肌动蛋白细胞骨架的实时成像对于许多基本生物学过程的研究至关重要，但目前可视化肌动蛋白的方法存在若干局限性。在此我们描述了Lifeact，一种由17个氨基酸组成的肽，它能对真核细胞和组织中的丝状肌动蛋白（F-肌动蛋白）结构进行染色。Lifeact在体外和体内均不干扰肌动蛋白动力学，并且其化学修饰的肽形式能够实现对不可转染细胞中肌动蛋白动力学的可视化。
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