生命荧光蛋白：一种用于可视化丝状肌动蛋白的多功能标记物。
Lifeact: a versatile marker to visualize F-actin.
作者信息
Riedl Julia, Crevenna Alvaro H, Kessenbrock Kai, Yu Jerry Haochen, Neukirchen Dorothee, Bista Michal, Bradke Frank, Jenne Dieter, Holak Tad A, Werb Zena, Sixt Michael, Wedlich-Soldner Roland
机构信息
Max Planck Institute of Biochemistry, Independent Junior Research Group Cellular Dynamics and Cell Patterning, Am Klopferspitz 18, 82152 Martinsried, Germany.
出版信息
Nat Methods. 2008 Jul;5(7):605-7. doi: 10.1038/nmeth.1220. Epub 2008 Jun 8.
Abstract
Live imaging of the actin cytoskeleton is crucial for the study of many fundamental biological processes, but current approaches to visualize actin have several limitations. Here we describe Lifeact, a 17-amino-acid peptide, which stained filamentous actin (F-actin) structures in eukaryotic cells and tissues. Lifeact did not interfere with actin dynamics in vitro and in vivo and in its chemically modified peptide form allowed visualization of actin dynamics in nontransfectable cells.
摘要
肌动蛋白细胞骨架的实时成像对于许多基本生物学过程的研究至关重要，但目前可视化肌动蛋白的方法存在若干局限性。在此我们描述了Lifeact，一种由17个氨基酸组成的肽，它能对真核细胞和组织中的丝状肌动蛋白（F-肌动蛋白）结构进行染色。Lifeact在体外和体内均不干扰肌动蛋白动力学，并且其化学修饰的肽形式能够实现对不可转染细胞中肌动蛋白动力学的可视化。
相似文献
1
Lifeact: a versatile marker to visualize F-actin.
Nat Methods. 2008 Jul;5(7):605-7. doi: 10.1038/nmeth.1220. Epub 2008 Jun 8.
2
Lifeact mice for studying F-actin dynamics.
Nat Methods. 2010 Mar;7(3):168-9. doi: 10.1038/nmeth0310-168.
3
Application of Lifeact reveals F-actin dynamics in Arabidopsis thaliana and the liverwort, Marchantia polymorpha.
Plant Cell Physiol. 2009 Jun;50(6):1041-8. doi: 10.1093/pcp/pcp055. Epub 2009 Apr 15.
4
Actin fusion proteins alter the dynamics of mechanically induced cytoskeleton rearrangement.
PLoS One. 2011;6(8):e22941. doi: 10.1371/journal.pone.0022941. Epub 2011 Aug 5.
5
Structure of the Lifeact-F-actin complex.
PLoS Biol. 2020 Nov 20;18(11):e3000925. doi: 10.1371/journal.pbio.3000925. eCollection 2020 Nov.
6
High expression of Lifeact in Arabidopsis thaliana reduces dynamic reorganization of actin filaments but does not affect plant development.
Cytoskeleton (Hoboken). 2011 Oct;68(10):578-87. doi: 10.1002/cm.20534. Epub 2011 Oct 4.
7
Lifeact cannot visualize some forms of stress-induced twisted F-actin.
Nat Methods. 2009 May;6(5):317. doi: 10.1038/nmeth0509-317.
8
Live-cell imaging of the cytoskeleton and mitochondrial-cytoskeletal interactions in budding yeast.
Methods Mol Biol. 2009;586:41-68. doi: 10.1007/978-1-60761-376-3_3.
9
F-actin dynamics in Neurospora crassa.
Eukaryot Cell. 2010 Apr;9(4):547-57. doi: 10.1128/EC.00253-09. Epub 2010 Feb 5.
10
Lifeact-mEGFP reveals a dynamic apical F-actin network in tip growing plant cells.
PLoS One. 2009 May 29;4(5):e5744. doi: 10.1371/journal.pone.0005744.
引用本文的文献
1
Mobilization of nuclear antiviral factors by exportin XPO1 via the actin network inhibits RNA virus replication.
PLoS Pathog. 2025 Aug 19;21(8):e1012841. doi: 10.1371/journal.ppat.1012841. eCollection 2025 Aug.
2
Unraveling the role of the WIPF1/ACTN4 complex in podosome formation of human placental EVTs: Insights into recurrent spontaneous abortion.
Genes Dis. 2025 May 2;12(6):101665. doi: 10.1016/j.gendis.2025.101665. eCollection 2025 Nov.
3
Hypotonic stimuli promote osteocyte dendrite formation by modulating actin dynamics via the TRPV4-CDC42 signaling pathway.
Mater Today Bio. 2025 Jul 21;34:102120. doi: 10.1016/j.mtbio.2025.102120. eCollection 2025 Oct.
4
ADPact: A versatile sensor for ADP-F-actin.
Proc Natl Acad Sci U S A. 2025 Jul 22;122(29):e2420467122. doi: 10.1073/pnas.2420467122. Epub 2025 Jul 16.
5
Migrating immune cells globally coordinate protrusive forces.
Nat Immunol. 2025 Jul 15. doi: 10.1038/s41590-025-02211-w.
6
Actin Retrograde Flow in Permeabilized Cells: Myosin-II Driven Centripetal Movement of Transverse Arcs.
Bio Protoc. 2016 Mar 5;6(5):e1743. doi: 10.21769/BioProtoc.1743.
7
Teach your microscope how to print: low-cost and rapid-iteration microfabrication for biology.
Lab Chip. 2025 Jul 14. doi: 10.1039/d5lc00181a.
8
Nonmuscle myosin II regulates presynaptic actin and neuronal mechanobiology in Drosophila.
J Cell Biol. 2025 Sep 1;224(9). doi: 10.1083/jcb.202501211. Epub 2025 Jul 11.
9
Interneuron migration defects during corticogenesis contribute to Dyrk1a haploinsufficiency syndrome pathogenesis.
Mol Psychiatry. 2025 Jul 10. doi: 10.1038/s41380-025-03109-7.
10
Group B Streptococci lyse endothelial cells to infect the brain in a zebrafish meningitis model.
PLoS Biol. 2025 Jul 3;23(7):e3003236. doi: 10.1371/journal.pbio.3003236. eCollection 2025 Jul.
本文引用的文献
1
Versatile fluorescent probes for actin filaments based on the actin-binding domain of utrophin.
Cell Motil Cytoskeleton. 2007 Nov;64(11):822-32. doi: 10.1002/cm.20226.
2
Visualizing cytoskeleton dynamics in mammalian cells using a humanized variant of monomeric red fluorescent protein.
FEBS Lett. 2006 May 1;580(10):2495-502. doi: 10.1016/j.febslet.2006.03.082. Epub 2006 Apr 17.
3
Deconstructing the cadherin-catenin-actin complex.
Cell. 2005 Dec 2;123(5):889-901. doi: 10.1016/j.cell.2005.09.020.
4
A contractile nuclear actin network drives chromosome congression in oocytes.
Nature. 2005 Aug 11;436(7052):812-8. doi: 10.1038/nature03810. Epub 2005 Jul 13.
5
Dynamic actin patterns and Arp2/3 assembly at the substrate-attached surface of motile cells.
Curr Biol. 2004 Jan 6;14(1):1-10. doi: 10.1016/j.cub.2003.12.005.
6
Actin cable dynamics in budding yeast.
Proc Natl Acad Sci U S A. 2002 Jan 22;99(2):751-6. doi: 10.1073/pnas.022462899.
7
Fluorescent speckle microscopy, a method to visualize the dynamics of protein assemblies in living cells.
Curr Biol. 1998 Nov 5;8(22):1227-30. doi: 10.1016/s0960-9822(07)00515-5.
8
Use of a fusion protein between GFP and an actin-binding domain to visualize transient filamentous-actin structures.
Curr Biol. 1998 Mar 26;8(7):405-8. doi: 10.1016/s0960-9822(98)70159-9.
9
Isolation and characterization of a novel actin filament-binding protein from Saccharomyces cerevisiae.
Oncogene. 1998 Jan 8;16(1):121-30. doi: 10.1038/sj.onc.1201487.
10
A role for Mac-1 (CDIIb/CD18) in immune complex-stimulated neutrophil function in vivo: Mac-1 deficiency abrogates sustained Fcgamma receptor-dependent neutrophil adhesion and complement-dependent proteinuria in acute glomerulonephritis.
J Exp Med. 1997 Dec 1;186(11):1853-63. doi: 10.1084/jem.186.11.1853.
Zotero 插件