Cardona-Arias Jaiberth Antonio, Carrasquilla-Agudelo Yoneida Elena, Restrepo-Posada Deisy Cristina
Grupo de investigación Salud y Sostenibilidad, Escuela de Microbiología, Universidad de Antioquia, Medellín, Colombia.
Rev Chilena Infectol. 2017 Feb;34(1):33-44. doi: 10.4067/S0716-10182017000100005.
The screening of neurocysticercosis is complex and immunological methods have varying validity.
To evaluate the validity of ELISA for antigen and antibody, and EITB for antibody in the screening of neurocysticercosis.
Meta-analysis of diagnostic tests with an ex-ante protocol implemented in five databases with 15 search strategies, ensuring reproducibility in the selection and extraction of information. Sensitivity, specificity, likelihood ratios (LR), diagnostic odds ratio and ROC curve were estimated in MetaDiSc, and predictive values, and Youden index were estimated in Epidat.
EITB presented sensitivity of 85.7% (95% CI 83.5-87.7), specificity 93.9% (95% CI = 92.7-95.0), PLR 19.6 (95% CI = 8,6-44.6), NLR 0.16 (95% CI = 0.12-0.21), OR diagnostic 136.2 (95% CI = 54.7-342.6) and area under the curve 0.926. In ELISA for antibody sensitivity was 87.5% (95% CI = 86.1-88.8), specificity 92.2% (95% CI = 91.4-93.0), PLR 11.3 (95% CI = 8.45-15.11), NLR 0.15 (95% CI = 0.13-0.18), diagnostic OR 87.4 (95% CI = 60.1-127.1) and area under the curve 0.950. ELISA for antigen showed low diagnostic validity. No differences were found in these parameters by sample, antigen or antibody type.
ELISA for antibodies and EITB have a similar diagnostic value, detection of serum and CSF showed a similar validity.
神经囊尾蚴病的筛查较为复杂，免疫方法的有效性各不相同。
评估酶联免疫吸附测定（ELISA）检测抗原和抗体以及酶联免疫电转印印迹法（EITB）检测抗体在神经囊尾蚴病筛查中的有效性。
采用事前方案对诊断试验进行荟萃分析，在五个数据库中运用15种检索策略，确保信息选择和提取的可重复性。在MetaDiSc中估计敏感性、特异性、似然比（LR）、诊断比值比和ROC曲线，在Epidat中估计预测值和尤登指数。
EITB的敏感性为85.7%（95%置信区间83.5 - 87.7），特异性为93.9%（95%置信区间 = 92.7 - 95.0），阳性似然比为19.6（95%置信区间 = 8.6 - 44.6），阴性似然比为0.16（95%置信区间 = 0.12 - 0.21），诊断比值比为136.2（95%置信区间 = 54.7 - 342.6），曲线下面积为0.926。ELISA检测抗体时，敏感性为87.5%（95%置信区间 = 86.1 - 88.8），特异性为92.2%（95%置信区间 = 91.4 - 93.0），阳性似然比为11.3（95%置信区间 = 8.45 - 15.11），阴性似然比为0.15（95%置信区间 = 0.13 - 0.18），诊断比值比为87.4（95%置信区间 = 60.1 - 127.1），曲线下面积为0.950。ELISA检测抗原显示出较低的诊断有效性。在这些参数方面，未发现样本、抗原或抗体类型存在差异。
ELISA检测抗体和EITB具有相似的诊断价值，检测血清和脑脊液显示出相似的有效性。
