Departamento de Inmunología, Instituto de Investigaciones Biomédicas, UNAM, Ciudad de México, Mexico.
Administración Nacional de Laboratorios e Institutos de Salud (ANLIS) "Dr. Carlos Malbrán", Buenos Aires, Argentina.
Parasitol Res. 2019 Oct;118(10):2891-2899. doi: 10.1007/s00436-019-06425-4. Epub 2019 Aug 15.
Immunodiagnosis has a supportive role in the diagnosis of neurocysticercosis (NCC). The aim of this study was to compare the validity of seven immunodiagnostic tests among serum samples from 58 patients with NCC, 26 patients with neurological diseases other than NCC, and 15 healthy controls. One test for viable parasite detection (HP10 antigen assay) and six for antibody detection were evaluated. For the entire sample, sensitivities ranged from 55.2% (NOVALISA) to 81.0% (enzyme-linked immunosorbent assay [ELISA] Taenia solium antibody), with the sensitivity of the latter test significantly higher than that of the in-house ELISA Taenia crassiceps, NOVALISA, enzyme-linked immunoelectrotransfer blot (EITB) CDC, and HP10. Overall, specificities were high, ranging from 85.4% (ELISA Ts) to 97.1% (NOVALISA), with no statistically significant differences. Detection of HP10 antigen was significantly associated with the presence of vesicular parasites. The simple and low-cost ELISA Taenia solium antibody Ab instead of EITB is recommended to support NCC diagnosis in both rural and hospital settings in Mexico.
免疫诊断在神经囊尾蚴病（NCC）的诊断中具有辅助作用。本研究旨在比较 58 例 NCC 患者、26 例非 NCC 神经系统疾病患者和 15 例健康对照者血清样本中 7 种免疫诊断检测方法的有效性。评估了一种用于检测活寄生虫的检测方法（HP10 抗原检测）和 6 种用于检测抗体的检测方法。在整个样本中，敏感性范围为 55.2%（NOVALISA）至 81.0%（酶联免疫吸附试验 [ELISA] 猪带绦虫抗体），后者的敏感性明显高于内部 ELISA 牛带绦虫、NOVALISA、酶联免疫电泳印迹（EITB）CDC 和 HP10。总体而言，特异性较高，范围为 85.4%（ELISA Ts）至 97.1%（NOVALISA），无统计学差异。HP10 抗原的检测与囊泡寄生虫的存在显著相关。建议在墨西哥的农村和医院环境中，使用简单且廉价的 ELISA 猪带绦虫抗体 Ab 替代 EITB 来支持 NCC 诊断。
