Cullinane A A, Rixon F J, Davison A J
Medical Research Council Virology Unit, Institute of Virology, U.K.
J Gen Virol. 1988 Jul;69 ( Pt 7):1575-90. doi: 10.1099/0022-1317-69-7-1575.
The genome structure of equine herpesvirus 1 (EHV-1) subtype 2 was shown by electron microscopic studies and restriction endonuclease site mapping to comprise two covalently linked segments (L, 109 kbp; S, 35 kbp). The S segment contains a unique sequence (US) flanked by a substantial inverted repeat (TRS/IRS). Thus, the genome structure of EHV-1 subtype 2 is similar to that published previously for EHV-1 subtype 1, but the two subtypes differ in the occurrences of EcoRI and BamHI restriction sites. Hybridization studies using cloned EHV-1 DNA showed that the genome of EHV-1 subtype 2 is colinear with the genomes of EHV-1 subtype 1 and herpes simplex virus type 1. DNA sequence data for four EHV-1 subtype 2 genes, including one potentially encoding a glycoprotein, were obtained by sequencing a 4574 bp BamHI fragment containing the junction between US and TRS. The genome structure, hybridization and sequence data confirm that EHV-1 subtype 2 is of the alphaherpesvirus lineage.
通过电子显微镜研究和限制性内切酶位点图谱分析表明，马疱疹病毒1型（EHV-1）2型亚型的基因组结构由两个共价连接的片段组成（L，109 kbp；S，35 kbp）。S片段包含一个独特序列（US），两侧有一个较大的反向重复序列（TRS/IRS）。因此，EHV-1 2型亚型的基因组结构与先前发表的EHV-1 1型亚型相似，但这两个亚型在EcoRI和BamHI限制性位点的出现情况上有所不同。使用克隆的EHV-1 DNA进行的杂交研究表明，EHV-1 2型亚型的基因组与EHV-1 1型亚型和单纯疱疹病毒1型的基因组呈共线性。通过对一个包含US和TRS之间连接处的4574 bp BamHI片段进行测序，获得了四个EHV-1 2型亚型基因的DNA序列数据，其中一个基因可能编码一种糖蛋白。基因组结构、杂交和序列数据证实EHV-1 2型亚型属于α疱疹病毒谱系。
