Schechter E M, Summers J, Ogston C W
Department of Immunology/Microbiology, Rush-Presbyterian-St. Luke's Medical Center, Chicago, Illinois 60612.
J Gen Virol. 1988 Jul;69 ( Pt 7):1591-9. doi: 10.1099/0022-1317-69-7-1591.
A DNA virus with the characteristics of a herpesvirus has been isolated from woodchuck hepatocytes cultured in vitro. We refer to this virus as herpesvirus of marmots (HVM). Electron microscopy of thin sections of HVM-infected cells showed nucleocapsids with a hexagonal outline and a diameter of 80 nm. Enveloped virions were seen in cytoplasmic vacuoles and outside the cell. Negatively stained virus particles purified from cell supernatants were enveloped with the characteristic appearance of herpesviruses. The DNA was double-stranded with a molecular size of approximately 140 kb and a G + C content of 73%. The virus replicated with a lytic effect in kidney cells of owl monkeys and African green monkeys, baby hamster kidney cells, feline kidney cells and WCH-17, a cell line derived from a woodchuck hepatoma. An indirect immunofluorescence assay has shown the presence of antibody to HVM in seven out of 37 animals tested. An important reason for studying HVM lies in its possible role in infection or the disease produced by woodchuck hepatitis virus, an animal model for human hepatitis B virus.
一种具有疱疹病毒特征的DNA病毒已从体外培养的土拨鼠肝细胞中分离出来。我们将这种病毒称为旱獭疱疹病毒（HVM）。对感染HVM的细胞进行超薄切片的电子显微镜观察显示，核衣壳呈六边形轮廓，直径为80纳米。在细胞质空泡中和细胞外可见有包膜的病毒粒子。从细胞上清液中纯化的经负染的病毒粒子具有疱疹病毒特有的外观。该DNA为双链，分子大小约为140 kb，G + C含量为73%。该病毒在夜猴和非洲绿猴的肾细胞、幼仓鼠肾细胞、猫肾细胞以及源自土拨鼠肝癌的WCH - 17细胞系中复制并产生裂解效应。间接免疫荧光试验表明，在37只受试动物中有7只存在抗HVM抗体。研究HVM的一个重要原因在于其可能在土拨鼠肝炎病毒（一种人类乙型肝炎病毒的动物模型）所引发的感染或疾病中发挥的作用。
