Jore J, De Geus B, Jackson R J, Pouwels P H, Enger-Valk B E
Medical Biological Laboratory TNO, Rijswijk, The Netherlands.
J Gen Virol. 1988 Jul;69 ( Pt 7):1627-36. doi: 10.1099/0022-1317-69-7-1627.
A transcription/translation system for generating poliovirus proteins in vitro has been used to assess the proteolytic activity of various polypeptides containing the virus-coded 3C region towards the poliovirus precursor protein P1. Plasmids containing a phage T7 promoter followed by either the complete poliovirus P1 sequence or various sequences containing the 3C region were used for this purpose. We showed that all except one of the 3C-containing polypeptides had a very restricted activity towards P1, generating only a small amount of VP1 and no VP0 or VP3. The only polypeptide capable of fully processing P1 into VP0, VP3 and VP1 in vitro was protein 3CD, consisting of the complete 3C and 3D sequences.
一种用于在体外生成脊髓灰质炎病毒蛋白的转录/翻译系统已被用于评估各种含有病毒编码的3C区域的多肽对脊髓灰质炎病毒前体蛋白P1的蛋白水解活性。为此，使用了含有噬菌体T7启动子，其后接完整的脊髓灰质炎病毒P1序列或各种含有3C区域的序列的质粒。我们发现，除一种含3C的多肽外，所有其他含3C的多肽对P1的活性都非常有限，仅产生少量VP1，而不产生VP0或VP3。唯一能够在体外将P1完全加工成VP0、VP3和VP1的多肽是由完整的3C和3D序列组成的蛋白3CD。
