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来自马拉维的一株非洲猪瘟病毒分离株的分子克隆与限制性内切酶图谱分析

Molecular cloning and restriction enzyme mapping of an African swine fever virus isolate from Malawi.

作者信息

Dixon L K

机构信息

Institute for Health, Woking, Surrey, U.K.

出版信息

J Gen Virol. 1988 Jul;69 ( Pt 7):1683-94. doi: 10.1099/0022-1317-69-7-1683.

DOI:10.1099/0022-1317-69-7-1683
PMID:2839602
Abstract

DNA prepared from a field isolate of African swine fever virus, which causes high mortality and severe disease in domestic pigs, was cloned in bacteriophage lambda and plasmid vectors. Clones containing DNA inserts overlapping with each other and together covering the complete genome, apart from short fragments close to the cross-linked termini of the genome, were obtained. A complete restriction enzyme site map of the genome for three enzymes was deduced.

摘要

从非洲猪瘟病毒的一个野外分离株提取的DNA（该病毒可在家猪中引起高死亡率和严重疾病）被克隆到噬菌体λ和质粒载体中。获得了含有彼此重叠且共同覆盖完整基因组（除了靠近基因组交联末端的短片段外）的DNA插入片段的克隆。推导了该基因组针对三种酶的完整限制性酶切位点图谱。

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