Linz U, Baker C C
Laboratory of Tumor Virus Biology, National Cancer Institute, Bethesda, Maryland 20892.
J Virol. 1988 Aug;62(8):2537-43. doi: 10.1128/JVI.62.8.2537-2543.1988.
Five of six bovine papillomavirus type 1 (BPV-1) promoters which were previously mapped by determining the 5' termini of viral mRNAs from bovine fibropapillomas and BPV-1-transformed cells were found to be active under in vitro transcription conditions. Transcription initiation at each of these promoters was accurate at the nucleotide level as determined by primer extension analysis. The most active promoter in vitro was P89, a typical RNA polymerase II promoter with both TATA and CAAT boxes. The promoters P7185 and P2443 also have TATA-like boxes, but none of the other promoters contain these typical regulatory elements.
通过确定来自牛纤维瘤和BPV-1转化细胞的病毒mRNA的5'末端，先前已绘制出六种1型牛乳头瘤病毒（BPV-1）启动子中的五种，发现在体外转录条件下它们具有活性。通过引物延伸分析确定，这些启动子中的每一个在核苷酸水平上的转录起始都是准确的。体外最活跃的启动子是P89，它是一个典型的具有TATA盒和CAAT盒的RNA聚合酶II启动子。启动子P7185和P2443也有类似TATA的盒，但其他启动子均不包含这些典型的调控元件。
