Dougherty J P, Temin H M
McArdle Laboratory, University of Wisconsin, Madison 53706.
J Virol. 1988 Aug;62(8):2817-22. doi: 10.1128/JVI.62.8.2817-2822.1988.
We recently described a protocol for determination of retrovirus mutation rates, that is, the mutation frequency in a single cycle of retrovirus replication (J.P. Dougherty and H.M. Temin, Mol. Cell. Biol. 6:4378-4395, 1987; J.P. Dougherty and H.M. Temin, p. 18-23, in J. H. Miller and M. P. Calos, ed., Gene Transfer Vectors for Mammalian Cells, 1987). We used this protocol to determine the mutation rates for defined mutations in a replicating retrovirus by using a spleen necrosis virus-based vector. We determined that the mutation rate for a single base pair substitution during replication of this avian retrovirus is 2 x 10(-5) per base pair per replication cycle and the insertion rate is 10(-7) per base pair per replication cycle. It will be possible to use this protocol to determine mutation rates for other retroviruses.
我们最近描述了一种用于测定逆转录病毒突变率的方法，即逆转录病毒单个复制周期中的突变频率（J.P. 多尔蒂和H.M. 特明，《分子细胞生物学》6:4378 - 4395，1987；J.P. 多尔蒂和H.M. 特明，第18 - 23页，载于J.H. 米勒和M.P. 卡洛编，《哺乳动物细胞基因转移载体》，1987）。我们使用该方法通过基于脾坏死病毒的载体来测定复制型逆转录病毒中特定突变的突变率。我们确定这种禽逆转录病毒复制过程中单个碱基对替换的突变率为每复制周期每碱基对2×10⁻⁵，插入率为每复制周期每碱基对10⁻⁷。使用该方法来测定其他逆转录病毒的突变率将成为可能。
