血管内皮生长因子（VEGF）基因-2549位点插入/缺失多态性与伊朗东南部女性子宫平滑肌瘤易感性相关。
The -2549 insertion/deletion polymorphism of VEGF gene associated with uterine leiomyoma susceptibility in women from Southeastern Iran.
作者信息
Keshavarzi Farshid, Salimi Saeedeh, Mohammadpour-Gharehbagh Abbas, Teimoori Batool, Yazdi Atefeh, Farajian-Mashhadi Farzaneh, Yaghmaei Minoo, Mokhtari Mojgan
机构信息
Department of Clinical Biochemistry, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran.
出版信息
Ginekol Pol. 2017;88(3):115-119. doi: 10.5603/GP.a2017.0022.
OBJECTIVES
Vascular endothelial growth factor (VEGF) is an important angiogenic factor that regulates angiogenesis and mediates sex steroid-induced cell growth. The present study investigated the association of VEGF gene-2578C/A (rs699947) and -2549 insertion/deletion polymorphisms in the promoter region of VEGF-A gene and uterine leiomyoma susceptibility in Southeast of Iran.
MATERIAL AND METHODS
One hundred and fifty five women with uterine leiomyoma and 157 age, BMI, and ethnicity matched healthy women were enrolled in this study. VEGF gene -2578C/A polymorphism genotyping was performed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method, and the -2549 insertion/dele-tion polymorphism was analyzed by PCR method.
RESULTS
The frequency of alleles and genotypes of VEGF-2578C/A polymorphism was not different between women with uterine leiomyoma and the controls; however, a significant association was revealed between II genotype of -2549 insertion/deletion (I/D) polymorphism of VEGF gene and uterine leiomyoma.
CONCLUSIONS
The findings showed that VEGF gene -2549 insertion/deletion polymorphism was associated with uterine leiomyoma.
目的
血管内皮生长因子（VEGF）是一种重要的血管生成因子，可调节血管生成并介导性类固醇诱导的细胞生长。本研究调查了伊朗东南部VEGF基因-2578C/A（rs699947）和VEGF-A基因启动子区域-2549插入/缺失多态性与子宫肌瘤易感性之间的关联。
材料与方法
本研究纳入了155例子宫肌瘤女性患者以及157例年龄、BMI和种族相匹配的健康女性。采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法对VEGF基因-2578C/A多态性进行基因分型，采用PCR方法分析-2549插入/缺失多态性。
结果
子宫肌瘤女性患者与对照组之间VEGF-2578C/A多态性的等位基因和基因型频率无差异；然而，VEGF基因-2549插入/缺失（I/D）多态性的II基因型与子宫肌瘤之间存在显著关联。
结论
研究结果表明，VEGF基因-2549插入/缺失多态性与子宫肌瘤有关。
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