Kempinas W G, Lamano-Carvalho T L
Department of Stomatology, Faculty of Dentistry of Ribeirão Preto, SP, Brazil.
Lab Anim. 1988 Apr;22(2):154-6. doi: 10.1258/002367788780864547.
A method for estimating the concentration of spermatozoa in the rat cauda epididymidis is described. Treatment of a sperm suspension with 0.05% collagenase for 20-60 min or 0.025% trypsin for 1-2 min at 34-37 degrees C was found to result in consistently homogeneous sperm. Sperm concentration ranged from 152.5 to 230.0 X 10(7) spermatozoa/ml, with a mean of 187.7 (+/- 5.6 SEM) X 10(7) spermatozoa/ml.
描述了一种估计大鼠附睾尾部精子浓度的方法。发现在34 - 37摄氏度下，用0.05%胶原酶处理精子悬浮液20 - 60分钟或用0.025%胰蛋白酶处理1 - 2分钟，可使精子始终保持均匀状态。精子浓度范围为152.5至230.0×10⁷个精子/毫升，平均为187.7（±5.6标准误）×10⁷个精子/毫升。
