Lamano-Carvalho T L, Kempinas W G
Departamento de Estomatologia, Faculdade de Odontologia de Ribeirão Preto, Universidade de São Paulo, Brasil.
Braz J Med Biol Res. 1987;20(3-4):429-31.
A method for estimating the concentration of spermatozoa in the rat cauda epididymidis is described. Treatment of a sperm suspension with 0.05% collagenase for 20-60 min or 0.025% trypsin for 1-2 min at 34-37 degrees C was found to result in consistently homogeneous sperm. Sperm concentration ranged from 152.5 to 230.0 x 10(7) spermatozoa/ml, with a mean of 187.7 +/- 5.6 (SEM) x 10(7) spermatozoa/ml.
描述了一种估计大鼠附睾尾部精子浓度的方法。发现在34 - 37摄氏度下，用0.05%胶原酶处理精子悬浮液20 - 60分钟或用0.025%胰蛋白酶处理1 - 2分钟，可得到始终均匀的精子。精子浓度范围为152.5至230.0×10⁷个精子/毫升，平均值为187.7±5.6（标准误）×10⁷个精子/毫升。
