Borkhsenius S N, Chernov V M
Mol Biol (Mosk). 1988 Mar-Apr;22(2):439-45.
A family of DNA sequences (Sau3A repeat sequences) has been revealed in genomes of two forms of O. nerka. A DNA fragment belonging to this "family" has been cloned in bacterial plasmid. Its copy number (in genomes of normal (570 +/- 33) and dwarf (66 +/- 12) forms) has been counted. The structural organization of sequences of Sau3A family in genomes of males and females of normal form is not the same. The organization of Sau3A sequences is the same in genomes of dwarf males and dwarf females of normal form, but the quantity of Sau3A sequences copies is smaller. It is supposed that the DNA sequences of Sau3A family bear the sex determination of dwarf form of O. nerka. The fragment of rRNA gene of nerka has been cloned and the number of rRNA gene copies in genomes of normal (200 +/- 62) and dwarf (1690 +/- 24) forms has been defined.
在两种红大麻哈鱼的基因组中发现了一个DNA序列家族（Sau3A重复序列）。属于这个“家族”的一个DNA片段已被克隆到细菌质粒中。已计算出其拷贝数（在正常（570±33）和矮小型（66±12）形态的基因组中）。正常形态的雄性和雌性基因组中Sau3A家族序列的结构组织不同。正常形态的矮小型雄性和矮小型雌性基因组中Sau3A序列的组织相同，但Sau3A序列拷贝的数量较少。据推测，Sau3A家族的DNA序列决定了红大麻哈鱼矮小型的性别。已克隆了红大麻哈鱼的rRNA基因片段，并确定了正常（200±62）和矮小型（1690±24）形态基因组中rRNA基因的拷贝数。
