Tran Nhat-Thang, Alexandre-Gouabau Marie-Cécile, Pagniez Anthony, Ouguerram Khadija, Boquien Clair-Yves, Winer Norbert, Darmaun Dominique
INRA, UMR 1280, Physiology of Nutritional Adaptations, University of Nantes, IMAD and CRNH-Ouest, Nantes 44000, France.
Department of Gynecology and Obstetrics, Centre Hospitalier Universitaire Hotel-Dieu, Nantes 44000, France.
Nutrients. 2017 Apr 11;9(4):375. doi: 10.3390/nu9040375.
A low birth weight (LBW) leads to a higher risk of metabolic syndrome in adulthood. Literature suggests that citrulline supplementation in adulthood prevents the effect of a high fructose diet on energy metabolism. Whether neonatal citrulline supplementation would alter early growth or energy metabolism in the long-term in rats with LBW is unknown. LBW pups born from dams fed a low (4%) protein diet, were nursed by normally-fed dams and received isonitrogenous supplements of either l-citrulline or l-alanine by gavage from the sixth day of life until weaning, and were subsequently exposed to 10%-fructose in drinking water from weaning to 90 days of age. The oral glucose tolerance was tested (OGTT) at 70 days of age, and rats were sacrificed at 90 days of age. Pre-weaning citrulline supplementation failed to alter the growth trajectory, OGTT, plasma triglycerides, or fat mass accretion in adulthood; yet, it was associated with increased liver triglycerides, decreased liver total cholesterol, and a distinct liver lipidomic profile that may result in a predisposition to liver disease. We conclude that pre-weaning supplementation with citrulline does not impact early growth, but might impact liver fat metabolism in adulthood upon exposure to a high fructose diet.
低出生体重（LBW）会导致成年后患代谢综合征的风险增加。文献表明，成年期补充瓜氨酸可预防高果糖饮食对能量代谢的影响。新生儿补充瓜氨酸是否会长期改变低出生体重大鼠的早期生长或能量代谢尚不清楚。由喂食低蛋白（4%）饮食的母鼠所生的低出生体重幼崽，由正常喂食的母鼠哺乳，并从出生后第六天到断奶通过灌胃给予等氮补充剂，即L-瓜氨酸或L-丙氨酸，随后从断奶到90日龄饮用含10%果糖的水。在70日龄时进行口服葡萄糖耐量试验（OGTT），并在90日龄时处死大鼠。断奶前补充瓜氨酸未能改变成年后的生长轨迹、OGTT、血浆甘油三酯或脂肪量增加；然而，它与肝脏甘油三酯增加、肝脏总胆固醇降低以及可能导致肝病易感性的独特肝脏脂质组学特征有关。我们得出结论，断奶前补充瓜氨酸不会影响早期生长，但在成年后接触高果糖饮食时可能会影响肝脏脂肪代谢。
