自噬受体p62/SQSTM1的缺失提高了哺乳动物细胞中基因传递的效率。
Depletion of autophagy receptor p62/SQSTM1 enhances the efficiency of gene delivery in mammalian cells.
作者信息
Tsuchiya Megumi, Ogawa Hidesato, Koujin Takako, Kobayashi Shouhei, Mori Chie, Hiraoka Yasushi, Haraguchi Tokuko
机构信息
Graduate School of Frontier Biosciences, Osaka University, Suita, Japan.
Advanced ICT Research Institute Kobe, National Institute of Information and Communications Technology, Kobe, Japan.
出版信息
FEBS Lett. 2016 Aug;590(16):2671-80. doi: 10.1002/1873-3468.12262. Epub 2016 Jul 4.
Novel methods that increase the efficiency of gene delivery to cells will have many useful applications. Here, we report a simple approach involving depletion of p62/SQSTM1 to enhance the efficiency of gene delivery. The efficiency of reporter gene delivery was remarkably higher in p62-knockout murine embryonic fibroblast (MEF) cells compared with normal MEF cells. This higher efficiency was partially attenuated by ectopic expression of p62. Furthermore, siRNA-mediated knockdown of p62 clearly increased the efficiency of transfection of murine embryonic stem (mES) cells and human HeLa cells. These data indicate that p62 acts as a key regulator of gene delivery.
提高基因导入细胞效率的新方法将有许多有用的应用。在此，我们报告一种简单的方法，即通过消耗p62/SQSTM1来提高基因导入效率。与正常小鼠胚胎成纤维细胞（MEF）相比，报告基因在p62基因敲除的小鼠胚胎成纤维细胞中的导入效率显著更高。p62的异位表达部分减弱了这种更高的效率。此外，siRNA介导的p62敲低明显提高了小鼠胚胎干细胞（mES）和人宫颈癌细胞（HeLa）的转染效率。这些数据表明p62是基因导入的关键调节因子。
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