异硫氰酸荧光素（FITC）-葡聚糖外渗作为血脑屏障通透性的一种测量方法。
Fluorescein Isothiocyanate (FITC)-Dextran Extravasation as a Measure of Blood-Brain Barrier Permeability.
作者信息
Natarajan Reka, Northrop Nicole, Yamamoto Bryan
机构信息
Department of Pharmacology and Toxicology, Indiana University School of Medicine, Indianapolis, Indiana.
出版信息
Curr Protoc Neurosci. 2017 Apr 10;79:9.58.1-9.58.15. doi: 10.1002/cpns.25.
Abstract
The blood-brain barrier (BBB) is formed in part by vascular endothelial cells that constitute the capillaries and microvessels of the brain. The function of this barrier is to maintain homeostasis within the brain microenvironment and buffer the brain from changes in the periphery. A dysfunction of the BBB would permit circulating molecules and pathogens typically restricted to the periphery to enter the brain and interfere with normal brain function. As increased permeability of the BBB is associated with several neuropathologies, it is important to have a reliable and sensitive method that determines BBB permeability and the degree of BBB disruption. A detailed protocol is presented for assessing the integrity of the BBB by transcardial perfusion of a 10,000 Da FITC-labeled dextran molecule and its visualization to determine the degree of extravasation from brain microvessels. © 2017 by John Wiley & Sons, Inc.
摘要
血脑屏障（BBB）部分由构成脑毛细血管和微血管的血管内皮细胞形成。该屏障的功能是维持脑微环境内的稳态，并使大脑免受外周变化的影响。血脑屏障功能失调会使通常局限于外周的循环分子和病原体进入大脑并干扰正常脑功能。由于血脑屏障通透性增加与多种神经病理学相关，因此拥有一种可靠且灵敏的方法来测定血脑屏障通透性及血脑屏障破坏程度非常重要。本文介绍了一种详细方案，通过经心灌注10,000 Da异硫氰酸荧光素（FITC）标记的葡聚糖分子并对其进行可视化来评估血脑屏障的完整性，以确定从脑微血管渗出的程度。© 2017约翰威立国际出版公司
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