乌干达农村地区犬蚤（蚤目：角叶蚤科）中立克次氏体属的高流行率
High Prevalence of Rickettsia spp. in Dog Fleas (Siphonaptera: Pulicidae) in Rural Uganda.
作者信息
Palomar Ana M, Cevidanes Aitor, Portillo Aránzazu, Kalema-Zikusoka Gladis, Chirife Andrea D, Romero Lourdes, Muro Jesús, Mugisha Lawrence, Oteo José A, Millán Javier
机构信息
Center of Rickettsiosis and Arthropod-Borne Diseases, Hospital San Pedro-CIBIR, Logroño, Spain.
PhD Program in Conservation Medicine, Facultad de Ecología y Recursos Naturales, Universidad Andres Bello, Santiago, Chile.
出版信息
J Med Entomol. 2017 Jul 1;54(4):1076-1079. doi: 10.1093/jme/tjx048.
Fleas are known vectors of zoonotic agents. Thirty-five fleas, including 28 Ctenocephalides felis (Bouché), four Pulex irritans (L.), and three Echidnophaga gallinacea (Westwood) from 19 rural dogs from southwestern Uganda were analyzed for the presence of Rickettsia spp. (ompB, gltA, and 17 kDa fragment genes) and Bartonella spp. (rpoB and ITS genes) by PCR. Rickettsial DNA was detected in 27 out of 28 of Ct. felis and in two out of four P. irritans. None of the E. gallinacea specimens harbored Rickettsia DNA. Rickettsia felis was confirmed in 12 Ct. felis and in the two P. irritans specimens with positive PCR-results. In addition, the presence of Candidatus Rickettsia asemboensis was evidenced in 15 Ct. felis. Bartonella spp. was not amplified in any sample. Our survey indicates that R. felis, the agent of the flea-borne spotted fever, is present in the study area. Besides, this is the first description of Ca. R. asemboensis in Uganda.
跳蚤是已知的人畜共患病原体的传播媒介。对来自乌干达西南部19只农村犬身上的35只跳蚤进行了分析，其中包括28只猫栉首蚤（Bouché）、4只人蚤（L.）和3只禽刺蚤（Westwood），通过聚合酶链反应（PCR）检测立克次氏体属（ompB、gltA和17 kDa片段基因）和巴尔通体属（rpoB和ITS基因）的存在情况。在28只猫栉首蚤中的27只以及4只人蚤中的2只检测到立克次氏体DNA。所有禽刺蚤样本均未携带立克次氏体DNA。在12只猫栉首蚤以及2只PCR结果呈阳性的人蚤样本中确认存在猫立克次氏体。此外，在15只猫栉首蚤中证实存在类阿散波立克次氏体。在任何样本中均未扩增出巴尔通体属。我们的调查表明，蚤传斑点热病原体猫立克次氏体存在于研究区域。此外，这是乌干达首次对类阿散波立克次氏体的描述。
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