厄瓜多尔从狗身上采集的猫栉首蚤和致痒蚤中发现的“类阿森博埃立克次体”及沃尔巴克氏体属细菌
'Candidatus Rickettsia asemboensis' and Wolbachia spp. in Ctenocephalides felis and Pulex irritans fleas removed from dogs in Ecuador.
作者信息
Oteo José A, Portillo Aránzazu, Portero Francisco, Zavala-Castro Jorge, Venzal José M, Labruna Marcelo B
机构信息
Departamento de Enfermedades Infecciosas, Hospital San Pedro-Centro de Investigación Biomédica de La Rioja (CIBIR), C/ Piqueras 98, Logroño (La Rioja), 26006, Spain.
出版信息
Parasit Vectors. 2014 Sep 30;7:455. doi: 10.1186/s13071-014-0455-0.
BACKGROUND
Flea-borne infections are distributed worldwide. Up to date there are no reports about microorganisms associated to fleas in Ecuador.
METHODS
Seventy-one Pulex irritans and 8 Ctenocephalides felis fleas were removed from dogs in two Ecuadorian areas (Pastaza and Chimborazo Provinces) in December 2012. DNA extracts were tested by polymerase chain reaction (PCR) assays targeting universal 16S rRNA, as well as screened for the presence of Rickettsia spp. (gltA, htrA, ompB, sca4 and ompA genes) and Bartonella spp. (rpoB, gltA and ITS genes).
RESULTS
Our results showed the presence of 'Candidatus Rickettsia asemboensis' (highly similar to R. felis) in C. felis and Wolbachia spp. endosimbionts in P. irritans collected from animals in Ecuador. No fleas were found to be positive for any Bartonella species or Yersinia pestis.
CONCLUSIONS
Clinicians should be aware of the potential risk of this new Candidatus Rickettsia sp. and keep in mind other flea-borne infections since these flea species frequently bite humans.
背景
蚤媒感染在全球范围内均有分布。截至目前，厄瓜多尔尚无关于与跳蚤相关微生物的报道。
方法
2012年12月，从厄瓜多尔两个地区（帕斯塔萨省和钦博拉索省）的犬只身上采集了71只致痒蚤和8只猫栉首蚤。DNA提取物通过针对通用16S rRNA的聚合酶链反应（PCR）检测进行测试，并筛查立克次体属（gltA、htrA、ompB、sca4和ompA基因）和巴尔通体属（rpoB、gltA和ITS基因）的存在情况。
结果
我们的结果显示，在从厄瓜多尔动物身上采集的猫栉首蚤中存在“类阿桑博立克次体”（与猫立克次体高度相似），在致痒蚤中存在沃尔巴克氏体属内共生菌。未发现任何跳蚤对任何巴尔通体物种或鼠疫耶尔森菌呈阳性。
结论
临床医生应意识到这种新的类立克次体属的潜在风险，并记住其他蚤媒感染，因为这些跳蚤种类经常叮咬人类。
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