Department of Veterinary Disease Biology, Faculty of Health and Medical Sciences, University of Copenhagen, Stigbøjlen 4, DK-1870, Frederiksberg C, Denmark; Key Laboratory of Freshwater Fishery Germplasm Resources, Ministry of Agriculture, Shanghai Ocean University, Shanghai, 201306, China.
Department of Veterinary Disease Biology, Faculty of Health and Medical Sciences, University of Copenhagen, Stigbøjlen 4, DK-1870, Frederiksberg C, Denmark.
Food Microbiol. 2017 Aug;65:19-24. doi: 10.1016/j.fm.2017.01.014. Epub 2017 Jan 31.
Integrated tilapia-pig farming, which uses manure from pigs as fertilizers in fish pond, is a traditional and common production system practised by small-scale farmers in South-east Asia. Although such systems may be environmentally sustainable, they also pose potential food safety hazards including transmission of faecal zoonotic pathogens and accumulation of antimicrobial and other chemical residues. This study aimed to determine differences in occurrence and characteristics of Salmonella spp. isolated from tilapia-pig and non-integrated aquaculture systems in Guangdong province, China. A total of 77 samples (9 pig feed, 19 fish feed, 9 pig faeces, 20 fish mucus and 20 fish intestine) from 10 tilapia-pig ponds and 10 non-integrated ponds were analysed. Salmonella spp. was found in fish mucus (20.0%), fish intestine (40.0%) and pig faeces (11.1%) from integrated ponds, and from fish mucus (40.0%) and fish intestine (40.0%) from non-integrated ponds. S. Weltevreden (76.5%) was by far the most common serovar showing limited antimicrobial resistance. One pig faeces sample contained S. Typhimurium whereas feed samples were found free of Salmonella spp.. DNA fingerprinting by the PFGE method showed a clonal relationship of S. Weltevreden which was supported by similar antimicrobial resistance patterns (sulfamethoxazole and trimethoprim resistance) as well as most isolates harbouring a 147-kb sized plasmid. The common finding of S. Weltevreden in both tilapia production systems indicates that this serovar may have a different ecology and increased survival in aquaculture environments in comparison with other Salmonella serovars. Further in vivo studies of the ecology of S. Weltevreden in aquaculture environments are needed.
罗非鱼-猪养殖一体化，即利用猪粪作为鱼塘肥料，是东南亚小农户采用的传统且常见的生产系统。尽管此类系统在环境方面具有可持续性，但也存在潜在的食品安全危害，包括粪源肠道动物病原体的传播以及抗生素和其他化学物质残留的积累。本研究旨在确定广东省罗非鱼-猪养殖一体化和非一体化养殖系统中分离的沙门氏菌的发生和特征差异。从广东省 10 个罗非鱼-猪养殖池塘和 10 个非一体化池塘中采集了 77 个样本（9 个猪饲料、19 个鱼饲料、9 个猪粪便、20 个鱼黏液和 20 个鱼肠）。在一体化池塘的鱼黏液（20.0%）、鱼肠（40.0%）和猪粪便（11.1%）中发现了沙门氏菌，而非一体化池塘的鱼黏液（40.0%）和鱼肠（40.0%）中也发现了沙门氏菌。到目前为止，最常见的血清型是 S. Weltevreden（76.5%），且表现出有限的抗生素耐药性。一个猪粪便样本中含有 S. Typhimurium，而饲料样本中未发现沙门氏菌。PFGE 方法的 DNA 指纹图谱显示 S. Weltevreden 具有克隆关系，这一关系得到了相似的抗生素耐药模式（磺胺甲恶唑和甲氧苄啶耐药）以及大多数分离株携带 147kb 大小质粒的支持。在两种罗非鱼生产系统中均发现 S. Weltevreden，表明与其他沙门氏菌血清型相比，该血清型可能在水产养殖环境中具有不同的生态和更高的生存能力。需要进一步研究 S. Weltevreden 在水产养殖环境中的生态学。
