SW16-7，一种具有高效裂解活性的新型噬菌体，靶向血清型。

SW16-7, a Novel Bacteriophage with Highly Effective Lytic Activity Targets Serovar .

作者信息

Xu Jialiang, Li Jia, Yan Yi, Han Pengjun, Tong Yigang, Li Xu

机构信息

China Food Flavor and Nutrition Health Innovation Center, Beijing Technology and Business University, Beijing 100048, China.

College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China.

出版信息

Microorganisms. 2023 Aug 15;11(8):2090. doi: 10.3390/microorganisms11082090.

DOI:10.3390/microorganisms11082090

PMID:37630650

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10458263/

Abstract

serovar is a foodborne pathogen commonly transmitted through fresh vegetables and seafood. In this study, a lytic phage, SW16-7, was isolated from medical sewage, demonstrating high infectivity against , , , and of Group O:3. In vitro inhibition assays revealed its effective antibacterial effect for up to 12 h. Moreover, analysis using the Comprehensive Antibiotic Resistance Database (CARD) and the Virulence Factor Database (VFDB) showed that SW16-7's genome does not contain any virulence factors or antibiotic resistance genes, indicating its potential as a promising biocontrol agent against . Additionally, a TSP gene cluster was identified in SW16-7's genome, with TSP1 and TSP2 showing a high similarity to lysogenic phages ε15 and ε34, respectively, in the C-terminal region. The whole-genome phylogenetic analysis classified SW16-7 within the family and indicated a close relationship with , which is consistent with the ANI results.

摘要

血清型是一种食源性病原体，通常通过新鲜蔬菜和海鲜传播。在本研究中，从医疗污水中分离出一种裂解性噬菌体SW16-7，它对O:3群的[具体菌名1]、[具体菌名2]、[具体菌名3]和[具体菌名4]具有高感染性。体外抑制试验表明其有效抗菌作用长达12小时。此外，使用综合抗生素抗性数据库（CARD）和毒力因子数据库（VFDB）进行的分析表明，SW16-7的基因组不包含任何毒力因子或抗生素抗性基因，这表明它有潜力成为一种有前景的针对[具体菌名]的生物防治剂。此外，在SW16-7的基因组中鉴定出一个TSP基因簇，其中TSP1和TSP2在C末端区域分别与溶原性噬菌体ε15和ε34具有高度相似性。全基因组系统发育分析将SW16-7归类于[噬菌体家族名]家族，并表明它与[相关噬菌体名]关系密切，这与ANI结果一致。