Zeng Hai Feng, Yan Shen, Wu Su Fan
Department of Plastic Surgery, Zhejiang Provincial People's Hospital, People's Hospital of Hangzhou Medical College, PR China.
Department of Plastic Surgery, Zhejiang Provincial People's Hospital, People's Hospital of Hangzhou Medical College, PR China.
Biochem Biophys Res Commun. 2017 May 20;487(1):140-145. doi: 10.1016/j.bbrc.2017.04.032. Epub 2017 Apr 8.
Malignant melanoma is one of the most common malignancies of the skin cancer and increasing evidences revealed that microRNAs (miRNAs) exert significant effects in melanoma. In the present study, the underlying function of microRNA-153-3p (miR-153-3p) in melanoma was investigated from different levels, including cell level, protein level and gene level. Our results showed that expression of miR-153-3p was lower in melanoma tissues and melanoma cells compared with the para-tumor tissue and normal melanocytes. The overexpression of miR-153-3p inhibited the cell proliferation and invasion, at the same time promoted cell apoptosis. Moreover, we identified that snail family transcriptional repressor 1 (SNAI1) is the direct target of miR-153-3p, and there is a negative correlation between miR-153-3p level and SNAI1 expression. In summary, we presented the evidences that miR-153-3p may act as a tumor suppressor by down-regulating the expression of SNAI1 in melanoma and miR-153-3p might be a potential biomarker in the diagnosis and treatment of malignant melanoma.
恶性黑色素瘤是皮肤癌中最常见的恶性肿瘤之一，越来越多的证据表明，微小RNA（miRNA）在黑色素瘤中发挥着重要作用。在本研究中，我们从细胞水平、蛋白质水平和基因水平等不同层面研究了微小RNA-153-3p（miR-153-3p）在黑色素瘤中的潜在功能。我们的研究结果表明，与癌旁组织和正常黑素细胞相比，miR-153-3p在黑色素瘤组织和黑色素瘤细胞中的表达较低。miR-153-3p的过表达抑制了细胞增殖和侵袭，同时促进了细胞凋亡。此外，我们确定蜗牛家族转录抑制因子1（SNAI1）是miR-153-3p的直接靶点，并且miR-153-3p水平与SNAI1表达之间存在负相关。总之，我们提供的证据表明，miR-153-3p可能通过下调SNAI1在黑色素瘤中的表达而发挥肿瘤抑制作用，并且miR-153-3p可能是恶性黑色素瘤诊断和治疗中的一个潜在生物标志物。
