Department of Pharmacy, School of Medicine, University of Naples Federico II, Naples, Italy.
Department of Experimental Oncology, National Cancer Institute, G. Pascale, Naples, Italy.
Biochem Pharmacol. 2018 Oct;156:52-59. doi: 10.1016/j.bcp.2018.08.008. Epub 2018 Aug 9.
Malignant melanoma is one of the most leading form of skin cancer associated with a low patient survival rate. Increasing evidence revealed that microRNAs (miRNAs) play a crucial role in the occurrence and development of several form of cancer including melanoma. In this study, we aimed at investigating the expression and role of miR-143-3p in human malignant melanoma. Our results showed that the expression of miR-143-3p was lower in human melanoma cells, as well as human biopsy specimens, when compared to normal human melanocytes. Ectopic expression of miR-143-3p in human melanoma cells inhibited proliferation, migration, invasion and promoted apoptosis acting through a molecular mechanism that, at least in part, is dependent on inhibition of cyclooxygenase-2 (COX-2) gene. Collectively, these results demonstrate that miR-143-3p could represent at the same time, a new early diagnostic marker and therapeutic target acting as tumor suppressor in melanoma cancer.
恶性黑色素瘤是与低患者生存率相关的最主要皮肤癌形式之一。越来越多的证据表明，microRNAs（miRNAs）在包括黑色素瘤在内的几种癌症的发生和发展中起着关键作用。在这项研究中，我们旨在研究 miR-143-3p 在人恶性黑色素瘤中的表达和作用。我们的结果表明，与正常人黑素细胞相比，miR-143-3p 在人黑色素瘤细胞和人活检标本中的表达较低。miR-143-3p 在人黑色素瘤细胞中的异位表达通过一种分子机制抑制增殖、迁移、侵袭，并促进凋亡，至少部分依赖于对环氧化酶-2（COX-2）基因的抑制。总之，这些结果表明，miR-143-3p 既可以作为早期诊断标志物，又可以作为肿瘤抑制因子在黑色素瘤中发挥作用的治疗靶点。
