伊朗伊斯法罕省萨达特人群与非萨达特人群中Y染色体短串联重复序列标记频率的比较。
Comparison of the Frequency of Y-short Tandem Repeats Markers between Sadat and Non-Sadat Populations in Isfahan Province of Iran.
作者信息
Seyedebrahimi Reihaneh, Esfandiari Ebrahim, Rashidi Bahman, Salehi Rasoul, Dahghi Ali Gholami, Dabiri Shahriar, Kheirollahi Majid
机构信息
Department of Anatomy, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
Department of Genetics and Molecular Biology, Research Institute for Primordial Prevention of Noncommunicable Disease, Isfahan University of Medical Sciences, Isfahan, Iran; Pediatric Inherited Diseases Research Center, Research Institute for Primordial Prevention of Noncommunicable Disease, Isfahan University of Medical Sciences, Isfahan, Iran.
出版信息
Adv Biomed Res. 2017 Mar 7;6:33. doi: 10.4103/2277-9175.188493. eCollection 2017.
BACKGROUND
Y chromosome is one of the two sex chromosomes and is male specific. Due to limited genetic exchange, the main part of that is passed virtually unchanged from one generation to next generation. The short tandem repeats (STRs) are almost constant on chromosomes that make them as an appropriate factor for use in population genetic studies. In this study, we used the STRs of Y chromosome markers in Sadat families and comparison with other families was investigated.
MATERIALS AND METHODS
In this study, sampling was done from fifty unrelated males of Sadat families and fifty unrelated males of non-Sadat families. After the extraction of DNA from blood samples and primer design, polymerase chain reaction (PCR) was performed for each primer pairs separately. The PCR products were run on agarose gel that followed by running on polyacrylamide gel for better resolution. In addition, some sequenced samples were used as identified markers to determine the length of other alleles in polyacrylamide gel.
RESULTS
The survey of six STR in two case and control groups was carried out, and analysis revealed that the frequency of some alleles is different in case group compared to control group. Allele frequency of the markers DYS392, DYS393, DYS19, DYS390, DYS388, and DYS437 on the Y chromosome in Sadat families was quite different in comparison with other families.
CONCLUSIONS
The reason for these differences in allele frequencies of the Sadat family in comparison with other families is having a common ancestor.
背景
Y染色体是两条性染色体之一，具有男性特异性。由于基因交换有限，其主要部分几乎代代相传保持不变。短串联重复序列（STRs）在染色体上几乎是恒定的，这使其成为群体遗传学研究中一个合适的因素。在本研究中，我们使用了萨达特家族Y染色体标记的STRs，并与其他家族进行了比较研究。
材料与方法
在本研究中，从50名萨达特家族无关男性和50名非萨达特家族无关男性中进行采样。从血样中提取DNA并设计引物后，分别对每对引物进行聚合酶链反应（PCR）。PCR产物在琼脂糖凝胶上进行电泳，随后在聚丙烯酰胺凝胶上进行电泳以获得更好的分辨率。此外，一些测序样本用作鉴定标记，以确定聚丙烯酰胺凝胶中其他等位基因的长度。
结果
对两个病例组和对照组中的六个STR进行了检测，分析显示病例组中一些等位基因的频率与对照组不同。与其他家族相比，萨达特家族Y染色体上标记DYS392、DYS393、DYS19、DYS390、DYS388和DYS437的等位基因频率有很大差异。
结论
与其他家族相比，萨达特家族等位基因频率存在差异的原因是拥有共同祖先。
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