Park Seon-Joo, Kim Mi-Ok, Kim Jung Hoan, Jeong Sehyun, Kim Min Hee, Yang Su-Jin, Lee Jongsung, Lee Hae-Jeung
Department of Food and Nutrition, College of BioNano Technology, Gachon University, Gyeonggi 13120, Korea.
Department of Genetic Engineering, College of Biotechnology and Bioengineering, Sungkyunkwan University, Gyeonggi 16419, Korea.
Prev Nutr Food Sci. 2017 Mar;22(1):16-20. doi: 10.3746/pnf.2017.22.1.16. Epub 2017 Mar 31.
This study investigated the antioxidant activity of functional beverage concentrates containing herbal medicine extracts (FBCH) using various antioxidant assays, such as 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radical scavenging activity, and reducing power assay. The total polyphenolic content of FBCH (81.45 mg/100 g) was higher than Ssanghwa tea (SHT, 37.56 mg/100 g). The antioxidant activities of FBCH showed 52.92% DPPH and 55.18% ABTS radical scavenging activities at 100 mg/mL, respectively. FBCH showed significantly higher antioxidant activities compared to the SHT (DPPH, 23.43%; ABTS, 22.21%; reducing power optical density; 0.23, <0.05). In addition, intracellular reactive oxygen species generation significantly decreased in a concentration-dependent manner following FBCH treatment. These results suggest that the addition of herbal medicine extract contributes to the improved functionality of beverage concentrates.
本研究使用多种抗氧化测定方法,如1,1-二苯基-2-苦基肼(DPPH)自由基清除活性、2,2'-联氮-双-(3-乙基苯并噻唑啉-6-磺酸)(ABTS)自由基清除活性和还原能力测定,研究了含有草药提取物的功能性饮料浓缩物(FBCH)的抗氧化活性。FBCH的总多酚含量(81.45毫克/100克)高于双花茶(SHT,37.56毫克/100克)。FBCH的抗氧化活性在100毫克/毫升时分别显示出52.92%的DPPH和55.18%的ABTS自由基清除活性。与SHT相比,FBCH显示出显著更高的抗氧化活性(DPPH,23.43%;ABTS,22.21%;还原能力光密度;0.23,<0.05)。此外,FBCH处理后,细胞内活性氧的产生以浓度依赖性方式显著降低。这些结果表明,添加草药提取物有助于提高饮料浓缩物的功能。