Joshi D, Naidu P S, Singh A, Kulkarni S K
Pharmacology Division, University Institute of Pharmaceutical Sciences, Panjab University, Chandigarh, India.
J Med Food. 2005 Fall;8(3):392-6. doi: 10.1089/jmf.2005.8.392.
Chronic administration of ethanol (2 g/kg, p.o.) on days 1-6 and its withdrawal produced an anxiogenic reaction in mice as assessed in the mirrored-chamber test. Daily administration of quercetin (25 or 50 mg/kg, p.o.) prior to ethanol for 6 days prevented withdrawal-induced anxiety in mice. However, acute administration of a single dose of quercetin (50 mg/kg) to animals withdrawn from ethanol, i.e., on day 7, did not prevent withdrawal-induced anxiety. Ethanol withdrawal also induced a significant increase in the locomotor activity of mice indicating an anxiogenic response. Daily administration of quercetin (25 or 50 mg/kg, p.o.) prior to ethanol for 6 days prevented withdrawal-induced increased locomotor activity. Ethanol withdrawal also sensitized the convulsogenic reaction to pentylenetetrazole (PTZ). A non-convulsive dose (40-60 mg/kg) of PTZ produced full-blown convulsions and increased mortality in ethanol-withdrawn mice. Both acute and chronic administration of quercetin (25 or 50 mg/kg, p.o.) produced a significant protection against ethanol withdrawal-induced reduction in PTZ threshold in mice. The result suggests the protective effect of this safe drug, quercetin, in the management of ethanol withdrawal reactions.
在第1 - 6天对小鼠口服给予乙醇（2克/千克）并随后撤药，通过镜像箱试验评估发现这会在小鼠中产生致焦虑反应。在乙醇给药前6天每天口服给予槲皮素（25或50毫克/千克）可预防小鼠撤药诱导的焦虑。然而，在撤药后的第7天，即对已撤去乙醇的动物急性给予单剂量的槲皮素（50毫克/千克），并不能预防撤药诱导的焦虑。乙醇撤药还导致小鼠运动活动显著增加，表明存在致焦虑反应。在乙醇给药前6天每天口服给予槲皮素（25或50毫克/千克）可预防撤药诱导的运动活动增加。乙醇撤药还使小鼠对戊四氮（PTZ）的惊厥反应敏感化。非惊厥剂量（40 - 60毫克/千克）的PTZ在撤去乙醇的小鼠中可引发全面惊厥并增加死亡率。急性和慢性口服给予槲皮素（25或50毫克/千克）均可显著保护小鼠免受乙醇撤药诱导的PTZ阈值降低的影响。结果表明这种安全药物槲皮素在管理乙醇撤药反应方面具有保护作用。
