Wang Qing, Ding Gang, Xu Xin
Department of Implantology, Shandong Provincial Key Laboratory of Oral Biomedicine, Shandong University School of Stomatology, Jinan250012, Republic of China; Department of Dentistry, Weifang People's Hospital, Weifang261000, Shandong Province, People's Republic of China.
Department of Stomatology, Yidu Central Hospital, Weifang Medical University, Linglongshan South Road No.4138, Qingzhou262500, Shandong Province, People's Republic of China.
Open Med (Wars). 2017 Mar 6;12:19-23. doi: 10.1515/med-2017-0004. eCollection 2017 Jan.
Periodontal ligament stem cells (PDLSCs) are promising cell resource for the cell-based therapy for periodontitis and regeneration of bio-root. In this study, we investigated the effect of PDLSCs on neutrophil, a critical constituent of innate immunity, and the underlying mechanisms. The effect of PDLSCs on the proliferation and apoptosis of resting neutrophils and IL-8 activated neutrophils was tested under cell-cell contact culture and Transwell culture, with or without anti-IL-6 neutralizing antibody. We found that PDLSCs could promote the proliferation and reduce the apoptosis of neutrophils whether under cell-cell contact or Transwell culture. Anti-IL-6 antibody reduced PDLSCs-mediated inhibition of neutrophil apoptosis. IL-6 at the concentration of 10ng/ml and 20ng/ml could inhibit neutrophil apoptosis statistically. Collectively, PDLSCs could reduce the apoptosis of neutrophils via IL-6.
牙周膜干细胞（PDLSCs）是用于牙周炎细胞治疗和生物牙根再生的有前景的细胞资源。在本研究中，我们研究了PDLSCs对中性粒细胞（天然免疫的关键组成部分）的影响及其潜在机制。在细胞 - 细胞接触培养和Transwell培养条件下，使用或不使用抗IL - 6中和抗体，测试PDLSCs对静息中性粒细胞和IL - 8激活的中性粒细胞增殖和凋亡的影响。我们发现，无论在细胞 - 细胞接触还是Transwell培养条件下，PDLSCs都能促进中性粒细胞的增殖并减少其凋亡。抗IL - 6抗体减弱了PDLSCs介导的对中性粒细胞凋亡的抑制作用。浓度为10ng/ml和20ng/ml的IL - 6能在统计学上抑制中性粒细胞凋亡。总体而言，PDLSCs可通过IL - 6减少中性粒细胞的凋亡。
