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ACC脱氨酶、产铁载体及产氰荧光假单胞菌对特定作物的促生长作用

Crop specific plant growth promoting effects of ACCd enzyme and siderophore producing and cynogenic fluorescent Pseudomonas.

作者信息

Agrawal Toshy, Kotasthane Anil S, Kosharia Ashok, Kushwah Renu, Zaidi Najam Waris, Singh U S

机构信息

Department of Plant Pathology, Indira Gandhi Krishi Vishwavidyalaya, Krishak Nagar, Raipur, CG, 492006, India.

Department of Plant Molecular Biology and Biotechnology, Indira Gandhi Krishi Vishwavidyalaya, Krishak Nagar, Raipur, CG, 492006, India.

出版信息

3 Biotech. 2017 May;7(1):27. doi: 10.1007/s13205-017-0602-3. Epub 2017 Apr 11.

Abstract

Fluorescent Pseudomonas, aerobic, Gram-negative bacteria possess many traits that make them well suited as biocontrol and growth promoting agents. Our study revealed that isolates vary in mechanisms involved in the antagonist interactions against pathogen and growth stimulatory effects on host plant. Most of the potential antagonistic fluorescent Pseudomonas identified were avid iron chelators (P233, P201, 176, P76 and, P76). Wide variation in ACCd enzyme production was observed. ACCd enzyme assay tested P141 > P247 > P126, as potential ACCd enzyme producer. Cynogenic fluorescent Pseudomonas isolates P76 and P124 exerted strong inhibitory against S. rolfsii. However, another cynogenic fluorescent Pseudomonas P179 had no influence against R solani and S. rolfsii which remains unexplained. Noticeable crop specific plant growth stimulation exerted by different fluorescent Pseudomonas was observed on wheat (P124), chickpea (P72), lathyrus (P85, P216), greengram (P11), blackgram (P99, P233); bottlegourd (P248, P167); rice (P176, P247).

摘要

荧光假单胞菌是需氧革兰氏阴性菌,具有许多特性,使其非常适合作为生物防治和促进生长的制剂。我们的研究表明,分离株在与病原体的拮抗相互作用以及对宿主植物的生长刺激作用所涉及的机制方面存在差异。鉴定出的大多数潜在拮抗荧光假单胞菌都是 avid 铁螯合剂(P233、P201、176、P76 和 P76)。观察到 ACCd 酶产生存在广泛差异。ACCd 酶检测表明,作为潜在的 ACCd 酶生产者,P141 > P247 > P126。产氰荧光假单胞菌分离株 P76 和 P124 对罗氏白绢病菌具有强烈抑制作用。然而,另一种产氰荧光假单胞菌 P179 对茄丝核菌和罗氏白绢病菌没有影响,原因尚不明。观察到不同荧光假单胞菌对小麦(P124)、鹰嘴豆(P72)、山黧豆(P85、P216)、绿豆(P11)、黑豆(P99、P233);瓠瓜(P248、P167);水稻(P176、P247)有明显的作物特异性植物生长刺激作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e9b5/5388656/27cb10574926/13205_2017_602_Fig1_HTML.jpg

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