Mockevičiūtė Rima, Jurkonienė Sigita, Šveikauskas Vaidevutis, Zareyan Mariam, Jankovska-Bortkevič Elžbieta, Jankauskienė Jurga, Kozeko Liudmyla, Gavelienė Virgilija
Laboratory of Plant Physiology, Nature Research Centre, Akademijos Str. 2, 08412 Vilnius, Lithuania.
Department of Cell Biology and Anatomy, M.G. Kholodny Institute of Botany of the National Academy of Sciences of Ukraine, Tereshchenkivska Str. 2, 01601 Kyiv, Ukraine.
Plants (Basel). 2023 Mar 14;12(6):1301. doi: 10.3390/plants12061301.
In order to increase plants tolerance to drought, the idea of treating them with stress-protecting compounds exogenously is being considered. In this study, we aimed to evaluate and compare the impact of exogenous calcium, proline, and plant probiotics on the response of winter wheat to drought stress. The research was carried out under controlled conditions, simulating a prolonged drought from 6 to 18 days. Seedlings were treated with ProbioHumus 2 µL g for seed priming, 1 mL 100 mL for seedling spraying, and proline 1 mM according to the scheme. 70 g m CaCO was added to the soil. All tested compounds improved the prolonged drought tolerance of winter wheat. ProbioHumus, ProbioHumus + Ca had the greatest effect on maintaining the relative leaf water content (RWC) and in maintaining growth parameters close to those of irrigated plants. They delayed and reduced the stimulation of ethylene emission in drought-stressed leaves. Seedlings treated with ProbioHumus and ProbioHumus + Ca had a significantly lower degree of membrane damage induced by ROS. Molecular studies of drought-responsive genes revealed substantially lower expression of Ca and Probiotics + Ca treated plants vs. drought control. The results of this study showed that the use of probiotics in combination with Ca can activate defense reactions that can compensate for the adverse effects of drought stress.
为了提高植物的耐旱性，人们正在考虑用应激保护化合物对其进行外源处理的想法。在本研究中，我们旨在评估和比较外源钙、脯氨酸和植物益生菌对冬小麦干旱胁迫响应的影响。研究在可控条件下进行，模拟从6天到18天的长期干旱。按照方案，用2 μL/g的ProbioHumus进行种子引发处理，用1 mL/100 mL的ProbioHumus进行幼苗喷施处理，用1 mM的脯氨酸处理。向土壤中添加70 g/m²的CaCO₃。所有测试化合物均提高了冬小麦的长期耐旱性。ProbioHumus、ProbioHumus + Ca对维持叶片相对含水量（RWC）以及使生长参数接近灌溉植株的参数具有最大影响。它们延迟并减少了干旱胁迫叶片中乙烯释放的刺激。用ProbioHumus和ProbioHumus + Ca处理的幼苗由活性氧诱导的膜损伤程度显著较低。干旱响应基因的分子研究表明，与干旱对照相比，用Ca和益生菌 + Ca处理的植物的表达水平显著降低。本研究结果表明，将益生菌与Ca结合使用可以激活防御反应，从而弥补干旱胁迫的不利影响。
