Schönthal A, Herrlich P, Rahmsdorf H J, Ponta H
Kernforschungszentrum Karlsruhe, Universität Karlsruhe, Federal Republic of Germany.
Cell. 1988 Jul 29;54(3):325-34. doi: 10.1016/0092-8674(88)90195-x.
Transcription from the c-fos promoter and from minimal promoter constructs carrying the phorbol ester-responsive element [12-O-tetradecanoylphorbol-13-acetate (TPA) responsive element (TRE)] corresponding to the sequence in the human collagenase gene is activated by elevated levels of the oncogene products v-src, c-Ha-ras, activated c-Ha-ras, and v-mos, as well as by phorbol ester. Elevated c- or v-fos expression stimulates TRE-dependent transcription but represses the c-fos promoter. Antisense fos sequences abolish basal and induced transcription from TRE constructs and derepress the c-fos promoter. These results establish a key role for fos in signal transduction and implicate the fos protein as a trans-activating and -repressing molecule.
携带与人类胶原酶基因序列相对应的佛波酯反应元件[12 - O - 十四烷酰佛波醇 - 13 - 乙酸酯（TPA）反应元件（TRE）]的c - fos启动子和最小启动子构建体的转录，可被癌基因产物v - src、c - Ha - ras、活化的c - Ha - ras和v - mos的高水平激活，也可被佛波酯激活。c - fos或v - fos表达的升高会刺激TRE依赖性转录，但会抑制c - fos启动子。反义fos序列消除了TRE构建体的基础转录和诱导转录，并解除了对c - fos启动子的抑制。这些结果确立了fos在信号转导中的关键作用，并表明fos蛋白是一种反式激活和抑制分子。
