Department of Medical Biochemistry and Cell Biology, Institute of Biomedicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.
J Biol Chem. 2011 May 6;286(18):15619-24. doi: 10.1074/jbc.R111.233981. Epub 2011 Mar 1.
Replication of herpes simplex virus takes place in the cell nucleus and is carried out by a replisome composed of six viral proteins: the UL30-UL42 DNA polymerase, the UL5-UL8-UL52 helicase-primase, and the UL29 single-stranded DNA-binding protein ICP8. The replisome is loaded on origins of replication by the UL9 initiator origin-binding protein. Virus replication is intimately coupled to recombination and repair, often performed by cellular proteins. Here, we review new significant developments: the three-dimensional structures for the DNA polymerase, the polymerase accessory factor, and the single-stranded DNA-binding protein; the reconstitution of a functional replisome in vitro; the elucidation of the mechanism for activation of origins of DNA replication; the identification of cellular proteins actively involved in or responding to viral DNA replication; and the elucidation of requirements for formation of replication foci in the nucleus and effects on protein localization.
单纯疱疹病毒的复制发生在细胞核中，由一个由六个病毒蛋白组成的复制体来完成：UL30-UL42 DNA 聚合酶、UL5-UL8-UL52 解旋酶-引发酶和 UL29 单链 DNA 结合蛋白 ICP8。复制体通过 UL9 起始原点结合蛋白加载到复制原点上。病毒复制与重组和修复密切相关，通常由细胞蛋白完成。在这里，我们回顾了新的重要进展：DNA 聚合酶、聚合酶辅助因子和单链 DNA 结合蛋白的三维结构；体外重建功能复制体；阐明 DNA 复制原点激活的机制；鉴定积极参与或响应病毒 DNA 复制的细胞蛋白；阐明在核中形成复制焦点的要求以及对蛋白质定位的影响。
