Dpto. Química Inorgánica y Bioinorgánica, Facultad de Farmacia, UCM, Instituto de Investigación Sanitaria Hospital, 12 de Octubre i+12, 28040-Madrid, Spain.
Nanoscale. 2017 May 4;9(17):5528-5537. doi: 10.1039/c7nr01070b.
A new platform constituted by engineered responsive nanoparticles transported by human mesenchymal stem cells is here presented as a proof of concept. Ultrasound-responsive mesoporous silica nanoparticles are coated with polyethylenimine to favor their effective uptake by decidua-derived mesenchymal stem cells. The responsive-release ability of the designed nanoparticles is confirmed, both in vial and in vivo. In addition, this capability is maintained inside the cells used as carriers. The migration capacity of the nanoparticle-cell platform towards mammary tumors is assessed in vitro. The efficacy of this platform for anticancer therapy is shown against mammary tumor cells by inducing the release of doxorubicin only when the cell vehicles are exposed to ultrasound.
本文提出了一种由工程响应性纳米颗粒和人骨髓间充质干细胞构成的新平台，作为概念验证。超声响应介孔二氧化硅纳米颗粒用聚乙烯亚胺进行涂层处理，以促进其被蜕膜来源的间充质干细胞有效摄取。设计的纳米颗粒的响应释放能力在小瓶和体内均得到了证实。此外，这种能力在用作载体的细胞内得以保持。体外评估了纳米颗粒-细胞平台向乳腺肿瘤的迁移能力。通过仅在细胞载体暴露于超声时才释放阿霉素，该平台对乳腺肿瘤细胞的抗癌治疗效果得到了证明。
