Regeneration and Stem Cell Biology lab, Centre for Molecular and Nanomedical Sciences, International Research Centre, Sathyabama Institute of Science and Technology, Chennai, India.
Sci Data. 2024 Oct 7;11(1):1093. doi: 10.1038/s41597-024-03941-6.
Perionyx excavatus, an indigenous earthworm possesses exceptional regeneration capacity. Their anterior regeneration features wound closure, regeneration induction and morphogenesis of damaged organs. This study involved a complete analysis of their transcriptomic dataset, with an emphasis on identifying the genes expressed during regeneration and predicting their implications in the process of regeneration and morphogenesis. Control (first ten segments) and test (5 day blastema) RNA samples of biological replicates were isolated and sequenced on NovaSeq. 6000 using PE150 read length. An average of 98.64% of high-quality data was retained with assembly showing better continuity with the average transcript length with 823 bp and N50 value of 1,858 bp. This is the first report on the comparative transcriptome of P. excavatus during anterior regeneration and this study will shed light on the complexity of annelid regeneration.
挖掘沙蚕 Perionyx excavatus 是一种具有特殊再生能力的本地蚯蚓。它们的前体再生特征包括伤口闭合、再生诱导和受损器官的形态发生。本研究对其转录组数据集进行了全面分析，重点是鉴定在再生过程中表达的基因，并预测它们在再生和形态发生过程中的意义。从生物重复的对照（前 10 个体节）和测试（5 天的芽基）样本中分离 RNA，并使用 PE150 读长在 NovaSeq 6000 上进行测序。平均保留了 98.64%的高质量数据，组装结果显示具有更好的连续性，平均转录长度为 823bp，N50 值为 1858bp。这是关于 P. excavatus 在前端再生过程中的比较转录组的第一份报告，本研究将揭示环节动物再生的复杂性。
