Hughes K T, Roth J R
Department of Biology, University of Utah, Salt Lake City 84112.
Genetics. 1988 May;119(1):9-12. doi: 10.1093/genetics/119.1.9.
A genetic complementation system is described in which the complementing components are close together in a single linear DNA fragment; the complementation situation is temporary. This system is useful for providing transposition functions to transposition-defective transposons, since transposition functions act preferentially in cis. The basic procedure involves placing a transposition-defective transposon near the gene(s) for its transposition functions on a single DNA fragment. This fragment is introduced, here by general transduction, into a new host. The transposase acts in cis to permit the defective element to transpose from the introduced fragment into the recipient chromosome. The helper genes do not transpose and are lost by degradation and segregation. The method yields single insertion mutants that lack transposase and are not subject to further transposition or chromosome rearrangement. The general procedure is applicable to other sorts of transposable elements and could be modified for use in other genetic systems.
描述了一种基因互补系统，其中互补成分在单个线性DNA片段中紧密相邻；互补情况是暂时的。该系统可用于为转座缺陷型转座子提供转座功能，因为转座功能优先以顺式作用。基本步骤包括将转座缺陷型转座子置于单个DNA片段上其转座功能的基因附近。通过一般转导将该片段引入新宿主。转座酶以顺式作用使缺陷元件从引入的片段转座到受体染色体中。辅助基因不转座，通过降解和分离而丢失。该方法产生缺乏转座酶且不会发生进一步转座或染色体重排的单插入突变体。一般步骤适用于其他类型的转座元件，并且可以进行修改以用于其他遗传系统。
