Zulpo Dauton Luiz, Igarashi Michelle, Sammi Ana Sue, Santos Joeleni Rosa Dos, Sasse João Pedro, Cunha Ivo Alexandre Leme da, Taroda Alessandra, Barros Luiz Daniel de, Almeida Jonatas Campos de, Jenkins Mark Christopher, Navarro Italmar Teodorico, Garcia João Luis
Departamento de Medicina Veterinária Preventiva, Universidade Estadual de Londrina - UEL, Londrina, PR, Brasil.
Faculdade de Medicina Veterinária, Pontifícia Universidade Católica do Paraná - PUCPR, Toledo, PR, Brasil.
Rev Bras Parasitol Vet. 2017 Jan-Mar;26(1):67-73. doi: 10.1590/S1984-29612017007.
The aim of the present study was to evaluate oocyst shedding in cats immunized by nasal route with T. gondii proteins ROP2. Twelve short hair cats (Felis catus) were divided in three groups G1, G2 and G3 (n=4). Animals from G1 received 100 μg of rROP2 proteins plus 20 μg of Quil-A, G2 received 100 μg of BSA plus 20 μg of Quil-A, and the G3 only saline solution (control group). All treatments were done by intranasal route at days 0, 21, 42, and 63. The challenge was performed in all groups on day 70 with ≅ 800 tissue cysts of ME-49 strain by oral route. Animals from G1 shed less oocysts (86.7%) than control groups. ELISA was used to detect anti-rROP2 IgG and IgA, however, there were no correlation between number of oocyst shedding by either IgG or IgA antibody levels. In the present work, in spite of lesser oocysts production in immunized group than control groups, it was not possible to associate the use of rROP2 via nostrils with protection against oocyst shedding. For the future, the use of either other recombinant proteins or DNA vaccine, in combination with rROP2 could be tested to try improving the efficacy of this kind of vaccine.
本研究的目的是评估经鼻途径用弓形虫ROP2蛋白免疫的猫的卵囊排出情况。将12只短毛猫（家猫）分为G1、G2和G3三组（n = 4）。G1组动物接受100μg的rROP2蛋白加20μg的Quil-A，G2组接受100μg的牛血清白蛋白加20μg的Quil-A，G3组仅接受生理盐水（对照组）。所有处理均在第0、21、42和63天经鼻途径进行。在第70天，所有组通过口服途径用约800个ME-49株的组织包囊进行攻毒。G1组动物排出卵囊的数量（86.7%）少于对照组。采用酶联免疫吸附测定法检测抗rROP2 IgG和IgA，然而，卵囊排出数量与IgG或IgA抗体水平之间均无相关性。在本研究中，尽管免疫组产生的卵囊比对照组少，但无法将经鼻使用rROP2与防止卵囊排出联系起来。未来，可以测试使用其他重组蛋白或DNA疫苗与rROP2联合使用，以尝试提高这类疫苗的效力。
