Boyle D B, Coupar B E
Commonwealth Scientific and Industrial Research Organisation, Australian Animal Health Laboratory, Geelong, Victoria.
Gene. 1988 May 15;65(1):123-8. doi: 10.1016/0378-1119(88)90424-6.
Mycophenolic acid has been shown to be a potent inhibitor of vaccinia virus growth. By inserting the Escherichia coli xanthine-guanine phosphoribosyl transferase gene (gpt) into the vaccinia virus genome under control of the P-7.5 promoter this inhibition was overcome. When coupled in tandem with another gene of interest, recombinant vaccinia viruses can be positively selected carrying both genes. Since the gpt gene operates as a selectable marker in most mammalian cells it will be useful as a dominant selectable marker for the construction of recombinant viruses based on other host-specific poxviruses.
已证明霉酚酸是痘苗病毒生长的有效抑制剂。通过将大肠杆菌黄嘌呤 - 鸟嘌呤磷酸核糖基转移酶基因（gpt）插入到痘苗病毒基因组中P - 7.5启动子的控制下，这种抑制作用得以克服。当与另一个感兴趣的基因串联连接时，可以对携带这两个基因的重组痘苗病毒进行阳性选择。由于gpt基因在大多数哺乳动物细胞中作为选择标记起作用，因此它将作为基于其他宿主特异性痘病毒构建重组病毒的显性选择标记。
