大肠杆菌黄嘌呤-鸟嘌呤磷酸核糖基转移酶基因的核苷酸序列。
Nucleotide sequence of the Escherichia coli xanthine-guanine phosphoribosyl transferase gene.
作者信息
Pratt D, Subramani S
出版信息
Nucleic Acids Res. 1983 Dec 20;11(24):8817-23. doi: 10.1093/nar/11.24.8817.
Abstract
The Escherichia coli gene coding for the enzyme xanthine-guanine phosphoribosyl transferase (gpt) has been widely used as a dominant selectable marker in a variety of mammalian cells. We have determined the complete nucleotide sequence of the 1057 base pair (bp) segment of DNA containing this gene. The coding sequence for the enzyme is 456 nucleotides long and can code for a 152 amino acid (16.9 Kd) polypeptide. A comparison of the amino acid sequence of the bacterial enzyme with that of the mammalian hypoxanthine-guanine phosphoribosyl transferase (hprt) reveals no significant homology between the two polypeptides.
摘要
编码黄嘌呤 - 鸟嘌呤磷酸核糖基转移酶（gpt）的大肠杆菌基因已在多种哺乳动物细胞中广泛用作显性选择标记。我们已经确定了包含该基因的1057个碱基对（bp）DNA片段的完整核苷酸序列。该酶的编码序列长456个核苷酸，可编码一个152个氨基酸（16.9 Kd）的多肽。将该细菌酶的氨基酸序列与哺乳动物次黄嘌呤 - 鸟嘌呤磷酸核糖基转移酶（hprt）的氨基酸序列进行比较，发现这两种多肽之间没有明显的同源性。
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