Kalina M, Elmalek M, Hammel I
Department of Cell Biology and Histology, Sackler School of Medicine, Tel-Aviv University, Ramat-Aviv, Israel.
Histochemistry. 1988;89(2):193-8. doi: 10.1007/BF00489924.
Quantitative immunocytochemical studies were done by using the immunogold technique on sections of the intermediate lobe of rat pituitary. Antibodies raised (in rabbits) against the precursor proteins pro-opiomelanocortin (POMC) and ACTH were used. The results clearly indicate that the immature granules are the major site of POMC, as their antigenic density (gold beads/micron2) was almost 3 times as high as that of ACTH. In the mature granules, the antigenic density of ACTH was increased by 2.7-fold compared with the immature granules. Using a computer-assisted method, it was possible to categorize the granules' antigenic density according to their size. Using this approach it was found that the antigenic density of POMC remained constant in all mature granules of varied sizes, whereas the antigenic density of ACTH decreased with increasing granule size. The relationship between granule size, degree of maturation, and antigenic density is discussed.
采用免疫金技术对大鼠垂体中间叶切片进行了定量免疫细胞化学研究。使用了针对前体蛋白促肾上腺皮质激素原（POMC）和促肾上腺皮质激素（ACTH）（在兔体内产生）的抗体。结果清楚地表明，未成熟颗粒是POMC的主要位点，因为它们的抗原密度（金珠/微米²）几乎是ACTH的3倍。在成熟颗粒中，ACTH的抗原密度与未成熟颗粒相比增加了2.7倍。使用计算机辅助方法，可以根据颗粒大小对颗粒的抗原密度进行分类。采用这种方法发现，POMC的抗原密度在所有不同大小的成熟颗粒中保持恒定，而ACTH的抗原密度则随着颗粒大小的增加而降低。文中讨论了颗粒大小、成熟程度和抗原密度之间的关系。
