Lew S, Hammel I, Galli S J
Department of Pathology, Sackler School of Medicine, Tel-Aviv University, Ramat-Aviv, Israel.
Cell Tissue Res. 1994 Nov;278(2):327-36. doi: 10.1007/BF00414176.
We used a computer-assisted morphometry approach to analyze quantitatively the process of cytoplasmic granule formation in mouse pancreatic acinar cells stimulated with pilocarpine to induce secretion. Our findings suggest that each condensing vacuole/immature granule of pancreatic acinar cells is formed by the progressive aggregation of 106 to 128 "unit progranules" of narrowly fixed volume, define a range of 7.7 to 9.2 for the factor of volume condensation between the largest immature granules and the mature unit granule, and predict that the formation of a single mature unit granule by the aggregation and fusion of unit progranules involves a net reduction of at least 95% in the amount of membrane surface area associated with these structures.
我们采用计算机辅助形态测量方法，对用毛果芸香碱刺激以诱导分泌的小鼠胰腺腺泡细胞中细胞质颗粒形成过程进行了定量分析。我们的研究结果表明，胰腺腺泡细胞的每个浓缩泡/未成熟颗粒是由106至128个体积固定狭窄的“单位前颗粒”逐步聚集形成的，确定了最大未成熟颗粒与成熟单位颗粒之间体积浓缩因子的范围为7.7至9.2，并预测单位前颗粒通过聚集和融合形成单个成熟单位颗粒涉及与这些结构相关的膜表面积净减少至少95%。
