纳米富勒醇通过 NLRP3 炎性体和神经肽减轻根神经病。
Nanoparticle fullerol alleviates radiculopathy via NLRP3 inflammasome and neuropeptides.
机构信息
Department of Orthopaedic Surgery, University of Virginia, Charlottesville, VA, USA.
Department of Anesthesiology, University of Virginia, Charlottesville, VA, USA.
出版信息
Nanomedicine. 2017 Aug;13(6):2049-2059. doi: 10.1016/j.nano.2017.03.015. Epub 2017 Apr 9.
Abstract
The present study aimed to evaluate the analgesic effect of the antioxidant nanoparticle fullerol in a mouse radiculopathy and a dorsal root ganglion (DRG) culture models. Intervertebral disk degeneration causes significant hyperalgesia and nerve inflammation. Pain sensitization and inflammatory reaction were counteracted by fullerol when disk material was bathed in 10 or 100μM of fullerol prior to implantation. Immunohistochemistry showed similar massive IBA1 positive macrophage infiltration surrounding implanted disk material among groups, but IL-1β and IL-6 expression was decreased in the fullerol treated group. In the DRG explant culture, after treatment with TNF-α, the expression of IL-1β, NLRP3, and caspase 1 was significantly increased but this was reversed by the addition of fullerol. In addition, fullerol also decreased the expression of substance P and CGRP in the cultured DRGs. Nanoparticle fullerol effectively counteracts pain sensitization and the inflammatory cascade caused by disk degeneration.
摘要
本研究旨在评估抗氧化纳米粒子富勒醇在小鼠根性病变和背根神经节（DRG）培养模型中的镇痛作用。椎间盘退变引起明显的痛觉过敏和神经炎症。当椎间盘材料在植入前用 10 或 100μM 富勒醇浸泡时，富勒醇可对抗疼痛敏化和炎症反应。免疫组织化学显示，在各组中，围绕植入椎间盘材料的大量 IBA1 阳性巨噬细胞浸润相似，但富勒醇处理组的 IL-1β 和 IL-6 表达减少。在 DRG 外植体培养中，用 TNF-α处理后，IL-1β、NLRP3 和 caspase 1 的表达显著增加，但富勒醇的加入可逆转这种情况。此外，富勒醇还降低了培养的 DRGs 中 P 物质和 CGRP 的表达。纳米粒子富勒醇可有效对抗椎间盘退变引起的痛觉过敏和炎症级联反应。
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