Zuchowska A, Jastrzebska E, Zukowski K, Chudy M, Dybko A, Brzozka Z
Department of Microbioanalytics, Institute of Biotechnology, Warsaw University of Technology , Warsaw, Mazowieckie 00-664, Poland.
Biomicrofluidics. 2017 Mar 28;11(2):024110. doi: 10.1063/1.4979104. eCollection 2017 Mar.
In this paper, we present a culture of A549 and MRC-5 spheroids in a microfluidic system. The aim of our work was to develop a good lung cancer model for the evaluation of drug cytotoxicity. Our research was focused on determining the progress of cell aggregation depending on such factors as the depth of culture microwells in the microdevices, a different flow rate of the introduced cell suspensions, and the addition of collagen to cell suspensions. We showed that these factors had a significant influence on spheroid formation. It was found that both MRC-5 and A549 cells exhibited higher aggregation in 500 m microwells. We also noticed that collagen needs to be added to A549 cells to form the spheroids. Optimizing the mentioned parameters allowed us to form 3D lung tissue models in the microfluidic system during the 10-day culture. This study indicates how important an appropriate selection of the specified parameters is (e.g., geometry of the microwells in the microsystem) to obtain the spheroids characterized by high viability in the microfluidic system.
在本文中，我们展示了在微流控系统中培养A549和MRC-5球体的方法。我们工作的目的是开发一种用于评估药物细胞毒性的良好肺癌模型。我们的研究重点是根据诸如微器件中培养微孔的深度、引入的细胞悬液的不同流速以及向细胞悬液中添加胶原蛋白等因素来确定细胞聚集的进程。我们表明这些因素对球体形成有显著影响。发现MRC-5和A549细胞在500μm微孔中均表现出更高的聚集性。我们还注意到需要向A549细胞中添加胶原蛋白以形成球体。优化上述参数使我们能够在10天培养期间在微流控系统中形成三维肺组织模型。这项研究表明，为了在微流控系统中获得具有高活力特征的球体，适当选择特定参数（例如微系统中微孔的几何形状）是多么重要。
