Ren Y F, Alam S Q, Alam B S, Keefer L M
Department of Biochemistry and Molecular Biology, Louisiana State University Medical Center, New Orleans 70119.
Lipids. 1988 Apr;23(4):304-8. doi: 10.1007/BF02537338.
The effects of trans fatty acids on adenylate cyclase were determined in the submandibular salivary glands (SMSG) of rats fed diets containing 20% corn oil, 20% partially hydrogenated soybean oil (PHSBO) or 18% PHSBO + 2% corn oil. The fluoride- and isoproterenol-stimulated adenylate cyclase activities were higher in the SMSG from rats fed 20% PHSBO than in the control group fed 20% corn oil. The feeding of 2% corn oil with the diet containing 18% PHSBO resulted in a complete restoration of isoproterenol-stimulated and a partial restoration of fluoride-stimulated adenylate cyclase activity. There was no significant difference in the concentration of the beta-adrenergic receptor or in the receptor-binding affinity constants among the three dietary groups as measured by [3H]dihydroalprenolol (DHA)-binding. Higher fluorescence polarization of diphenyl hexatriene (DPH) was observed in SMSG membranes of rats fed diet containing 20% PHSBO than in the other two oils, suggesting that membrane fluidity may play a role in adenylate cyclase activity.
在喂食含20%玉米油、20%部分氢化大豆油（PHSBO）或18% PHSBO + 2%玉米油饮食的大鼠下颌下唾液腺（SMSG）中，测定了反式脂肪酸对腺苷酸环化酶的影响。喂食20% PHSBO的大鼠的SMSG中，氟化物和异丙肾上腺素刺激的腺苷酸环化酶活性高于喂食20%玉米油的对照组。在含18% PHSBO的饮食中添加2%玉米油进行喂食，导致异丙肾上腺素刺激的腺苷酸环化酶活性完全恢复，氟化物刺激的腺苷酸环化酶活性部分恢复。通过[3H]二氢阿普洛尔（DHA）结合测定，三个饮食组之间的β-肾上腺素能受体浓度或受体结合亲和常数没有显著差异。在喂食含20% PHSBO饮食的大鼠的SMSG膜中，观察到二苯基己三烯（DPH）的荧光偏振度高于其他两种油，这表明膜流动性可能在腺苷酸环化酶活性中起作用。
