Alam S Q, Alam B S
J Nutr. 1986 Sep;116(9):1620-30. doi: 10.1093/jn/116.9.1620.
The effects of a marginal and severe deficiency of essential fatty acids (EFA) on the fatty acid composition, fluorescence polarization of diphenylhexatriene (DPH) and adenylate cyclase activity were studied in plasma membranes of rat submandibular salivary glands (SMSG). Three groups of male weanling Sprague-Dawley rats were fed ad libitum EFA-deficient (EFAD), marginally EFA-deficient (MEFAD) or the control diets. Rats were killed after 8-17 wk of feeding and plasma membranes were prepared from the SMSG and the assayed for basal and fluoride-stimulated adenylate cyclase activity. In membranes prepared from EFAD and MEFAD rats, the fluoride-stimulated enzyme activity was 30-35% higher than that of the control group. Lower double-bond index of phospholipids in plasma membranes of the SMSG from EFAD and MEFAD rats was observed, suggesting a lower fluidity compared with the control group. Higher fluorescence polarization of DPH in membranes of EFAD and MEFAD rats was more evidence of lower fluidity. It is suggested that higher adenylate cyclase activity in SMSG of rats fed EFAD or MEFAD diets may be related to lower membrane fluidity. Fluoride-stimulated enzyme activity and fatty acid composition of membrane lipids were restored to normal values after feeding the control diets for 6 wk to the EFAD and MEFAD rats.
研究了必需脂肪酸（EFA）轻度和重度缺乏对大鼠下颌下唾液腺（SMSG）质膜中脂肪酸组成、二苯基己三烯（DPH）荧光偏振和腺苷酸环化酶活性的影响。三组雄性断乳Sprague-Dawley大鼠随意进食EFA缺乏（EFAD）、轻度EFA缺乏（MEFAD）或对照饮食。喂养8 - 17周后处死大鼠，从SMSG制备质膜，并测定基础和氟化物刺激的腺苷酸环化酶活性。在从EFAD和MEFAD大鼠制备的质膜中，氟化物刺激的酶活性比对照组高30 - 35%。观察到来自EFAD和MEFAD大鼠的SMSG质膜中磷脂的双键指数较低，表明与对照组相比流动性较低。EFAD和MEFAD大鼠质膜中DPH的荧光偏振较高，进一步证明了流动性较低。提示喂食EFAD或MEFAD饮食的大鼠SMSG中较高的腺苷酸环化酶活性可能与较低的膜流动性有关。在给EFAD和MEFAD大鼠喂食对照饮食6周后，氟化物刺激的酶活性和膜脂脂肪酸组成恢复到正常水平。
