Sugden B, Warren N
McArdle Laboratory for Cancer Research, University of Wisconsin-Madison 53706.
Mol Biol Med. 1988 Apr;5(2):85-94.
Two plasmids encoding resistance to hygromycin-B or to the analog of neomycin, G418, and containing either one or two copies of the plasmid origin of replication, oriP, of Epstein-Barr virus (EBV) were introduced into an EBV-positive B-lymphoblastoid cell line. Two clones of cells containing both plasmids were analyzed for the number of copies of each plasmid when the cells were propagated in the absence or in the presence of one or both selective agents. Under all conditions tested, the plasmid with two copies of oriP behaved in cells as did the plasmid with one copy of this plasmid origin of replication.
将两个编码对潮霉素 - B或新霉素类似物G418具有抗性且含有一或两个爱泼斯坦 - 巴尔病毒（EBV）质粒复制起点oriP拷贝的质粒导入一个EBV阳性的B淋巴母细胞系。当细胞在不存在或存在一种或两种选择剂的情况下传代培养时，对含有这两种质粒的两个细胞克隆进行了分析，以确定每种质粒的拷贝数。在所有测试条件下，含有两个oriP拷贝的质粒在细胞中的行为与含有该质粒复制起点一个拷贝的质粒相同。
