Perrine K G, Denker J A, Nilsen T W
Case Western Reserve University, School of Medicine, Department of Molecular Biology and Microbiology, Cleveland, OH 44106.
Mol Biochem Parasitol. 1988 Jul;30(1):97-104. doi: 10.1016/0166-6851(88)90137-5.
A genomic library of Brugia malayi was constructed and screened by hybridization with a cDNA clone corresponding to a potentially protective antigen of 63 kDa. The antigen is encoded by a multicopy gene family. Five distinct gene copies were isolated and one was characterized in detail by nucleotide sequence analysis. An apparent pseudogene was also characterized. The organization of genes encoding the antigen is typical of higher eukaryotes in exon/intron organization although the introns have an unusually high A+T content (75%). Organization of the genomic sequence along with S1 nuclease and primer extension analyses indicate that a short untranslated exon is spliced to the 5' end of the mRNAs encoding the antigen.
构建了马来布鲁线虫的基因组文库，并用与一个对应于63kDa潜在保护性抗原的cDNA克隆进行杂交筛选。该抗原由一个多拷贝基因家族编码。分离出五个不同的基因拷贝，并对其中一个进行了核苷酸序列分析的详细表征。还鉴定了一个明显的假基因。编码该抗原的基因组织在外显子/内含子组织方面是高等真核生物的典型特征，尽管内含子具有异常高的A+T含量（75%）。基因组序列的组织以及S1核酸酶和引物延伸分析表明，一个短的非翻译外显子被剪接到编码该抗原的mRNA的5'端。
