Ransohoff R M, Denker J A, Takacs A M, Hannon G J, Nilsen T W
Department of Molecular Biology and Microbiology, Case Western Reserve University School of Medicine, Cleveland, OH 44106.
Nucleic Acids Res. 1989 May 25;17(10):3773-82. doi: 10.1093/nar/17.10.3773.
DNA sequence analysis of genes encoding 5S rRNA in the human parasitic nematode Brugia malayi (B. malayi) indicates a surprising degree of heterogeneity. This variation in coding sequence is not accompanied by corresponding heterogeneity in flanking regions which are highly conserved. Six out of eight potential 5S coding regions differed; of these sequence variants, two were abundant in the B. malayi genome. Direct RNA sequence analysis indicated that one of these abundant variants accounts for most if not all of expressed 5S RNA at two stages of development.
对人类寄生线虫马来布鲁线虫（B. malayi）中编码5S rRNA的基因进行的DNA序列分析表明，其存在惊人程度的异质性。编码序列的这种变异并未伴随着侧翼区域相应的异质性，侧翼区域高度保守。八个潜在的5S编码区域中有六个不同；在这些序列变体中，有两个在马来布鲁线虫基因组中含量丰富。直接RNA序列分析表明，这些丰富变体中的一个在两个发育阶段占了大部分（如果不是全部）表达的5S RNA。
