KTH Royal Institute of Technology, School of Chemical Science and Engineering, Department of Chemistry, Analytical Chemistry, Stockholm, Sweden.
KTH Royal Institute of Technology, School of Chemical Science and Engineering, Department of Chemistry, Organic Chemistry, Stockholm, Sweden.
J Am Soc Mass Spectrom. 2017 Aug;28(8):1733-1736. doi: 10.1007/s13361-017-1657-4. Epub 2017 Apr 12.
Here we present a method to manufacture peptide-concentrating MALDI-plates with alkyl ketene dimer (AKD) as a new superhydrophobic coating. The fabrication of the hydrophobic plates included application of AKD by airbrush, and negative contact printing to generate the concentration sites. Deposited sample droplets were contained within the prestructured sites, and self-adjusted onto the site if slightly misplaced. No AKD contamination was observed, and the plates could easily be cleaned and regenerated. The S/N values for four model peptides was about twice as high compared with a standard steel plate and a commercial concentration plate. Graphical Abstract ᅟ.
在这里，我们提出了一种使用烷基烯酮二聚体（AKD）作为新型超疏水涂层来制造肽浓缩 MALDI 板的方法。疏水板的制造包括通过喷枪施加 AKD，并进行负接触印刷以产生浓缩点。沉积的样品液滴包含在预结构化的点内，如果稍微错位，它会自动调整到该点上。没有观察到 AKD 污染，并且可以轻松地清洁和再生这些板。与标准钢板和商业浓缩板相比，四种模型肽的 S/N 值大约提高了一倍。图摘要。
